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Wikipedia

OK cells

January 01, 1970

OK cells (short for opossum kidney cells) are a marsupial cell line used in medical research to model proximal tubule epithelial cells of the kidney.

Characteristics edit

The cell line was derived from the kidney of an adult female North American opossum (Didelphis virginiana).[1] Like porcine LLC-PK1 cells, this cell line has the limitation of lacking several enzymes specific to the proximal tubule.[2] Nonetheless, OK cells have been used extensively to study functional interactions between the parathyroid hormone 1 receptor (PTH1R) and the sodium-hydrogen exchange regulatory factor 1 (NHERF1).[3]

Use in research edit

OK cells were originally cultured as a source of X chromosomes for studies on X inactivation.[1] They have also served as models for the study of renal dopaminergic physiology, owing to their capacity to produce and degrade dopamine.[4] OK cells are sold under the catalog number CRL-1840 by ATCC.

References edit

  1. ^ a b Koyama, H.; Goodpasture, C.; Miller, M. M.; Teplitz, R. L.; Riggs, A. D. (March 1978). "Establishment and characterization of a cell line from the American opossum (Didelphys virginiana)". In Vitro. 14 (3): 239–246. doi:10.1007/BF02616032. PMID 566717. S2CID 27331795.
  2. ^ Kruidering, Marieke; Water, Bob van der; Nagelkerke, J. Fred (1996). Methods for Studying Renal Toxicity. Vol. 18. pp. 173–83. doi:10.1007/978-3-642-61105-6. ISBN 978-3-642-64696-6. PMID 8678793. S2CID 27034550. {{cite book}}: |journal= ignored (help)
  3. ^ Ciruela, Francisco; Luján, Rafael (2008). Molecular Aspects of G Protein-Coupled Receptors: Interacting Proteins and Function. New York, NY: Nova Science Publishers. p. 219. ISBN 9781600219153.
  4. ^ Guimarães, J.T.; et al. (April 1997). "Opossum kidney (OK) cells in culture synthesize and degrade the natriuretic hormone dopamine: A comparison with rat renal tubular cells". The International Journal of Biochemistry & Cell Biology. 29 (4): 681–688. doi:10.1016/S1357-2725(96)00166-5. PMID 9363646.

External links edit

  • Opossum Kidney Cortex Proximal Tubule Epithelial Cells (OK Line). Microscopy U. Retrieved February 15, 2015.
  • OK (ATCC® CRL-1840™). ATCC. Retrieved February 15, 2015.
  • Cellosaurus entry for OK

January 01, 1970
cells, short, opossum, kidney, cells, marsupial, cell, line, used, medical, research, model, proximal, tubule, epithelial, cells, kidney, contents, characteristics, research, references, external, linkscharacteristics, editthe, cell, line, derived, from, kidne. OK cells short for opossum kidney cells are a marsupial cell line used in medical research to model proximal tubule epithelial cells of the kidney Contents 1 Characteristics 2 Use in research 3 References 4 External linksCharacteristics editThe cell line was derived from the kidney of an adult female North American opossum Didelphis virginiana 1 Like porcine LLC PK1 cells this cell line has the limitation of lacking several enzymes specific to the proximal tubule 2 Nonetheless OK cells have been used extensively to study functional interactions between the parathyroid hormone 1 receptor PTH1R and the sodium hydrogen exchange regulatory factor 1 NHERF1 3 Use in research editOK cells were originally cultured as a source of X chromosomes for studies on X inactivation 1 They have also served as models for the study of renal dopaminergic physiology owing to their capacity to produce and degrade dopamine 4 OK cells are sold under the catalog number CRL 1840 by ATCC References edit a b Koyama H Goodpasture C Miller M M Teplitz R L Riggs A D March 1978 Establishment and characterization of a cell line from the American opossum Didelphys virginiana In Vitro 14 3 239 246 doi 10 1007 BF02616032 PMID 566717 S2CID 27331795 Kruidering Marieke Water Bob van der Nagelkerke J Fred 1996 Methods for Studying Renal Toxicity Vol 18 pp 173 83 doi 10 1007 978 3 642 61105 6 ISBN 978 3 642 64696 6 PMID 8678793 S2CID 27034550 a href Template Cite book html title Template Cite book cite book a journal ignored help Ciruela Francisco Lujan Rafael 2008 Molecular Aspects of G Protein Coupled Receptors Interacting Proteins and Function New York NY Nova Science Publishers p 219 ISBN 9781600219153 Guimaraes J T et al April 1997 Opossum kidney OK cells in culture synthesize and degrade the natriuretic hormone dopamine A comparison with rat renal tubular cells The International Journal of Biochemistry amp Cell Biology 29 4 681 688 doi 10 1016 S1357 2725 96 00166 5 PMID 9363646 External links editOpossum Kidney Cortex Proximal Tubule Epithelial Cells OK Line Microscopy U Retrieved February 15 2015 OK ATCC CRL 1840 ATCC Retrieved February 15 2015 Cellosaurus entry for OK Retrieved from https en wikipedia org w index php title OK cells amp oldid 1107914645, wikipedia, wiki, book, books, library,

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