A simple nitrite test can be performed by adding 4 M sulfuric acid to the sample until acidic, and then adding 0.1 M iron(II) sulfate to the solution. A positive test for nitrite is indicated by a dark brown solution, arising from the iron-nitric oxide complex ion. This test is related to the brown ring test for the nitrate ion, which forms the same complex in a ring. In contrast, nitrites turn the whole solution brown and therefore interfere with that test.[1]
A common method of quantitative nitrite detection is the Griess test, which relies on the reaction of nitrite with the two components of a Griess reagent to form a red azo dye. This allows the concentration of nitrite to be determined by UV-vis spectroscopy.[2]
Urinary nitrite testedit
A nitrite test is a standard component of a urinary test strip. A positive test for nitrites in the urine is called nitrituria.
This test is commonly used in diagnosing urinary tract infections (UTIs). A positive nitrite test indicates that the cause of the UTI is a Gram-negative organism, most commonly Escherichia coli. The reason for nitrites' existence in the presence of a UTI is due to a bacterial conversion of endogenous nitrates to nitrites. This may be a sign of infection. However, other parameters, such as leukocyte esterase, urine white blood cell count, and symptoms such as dysuria, urinary urgency, fevers, and chills must be correlated to diagnose an infection.[3]
Saliva testing; salivary nitrite levels can serve as a surrogate biomarker for nitric oxide, a natural cardioprotective factor, derived from nitrate-rich leafy green vegetables which are often found in anti-hypertensive diets, such as the DASH diet[5]
Referencesedit
^Holltzclaw, H.; Robinson, W. (1988). College Chemistry with qualitative analysis (8th ed.). Lexington, MA: D. C. Heath. pp. 1006–1007. OL 2415034M.
^Moorcroft, Matthew J.; Davis, James; Compton, Richard G. (2001). "Detection and Determination of Nitrate and Nitrite: A Review". Talanta. 54 (5): 785–803. doi:10.1016/S0039-9140(01)00323-X. PMID 18968301.
^Blood Cell Count - Control Your Health, Harold Amezcua, Health Testing Centers, September 12, 2015
^ abUrinary Tract Infection: Providing the Best Care. By Margaret A. Fitzgerald. Medical Writer: Sandra M. Nettina. Published: 06/24/2002; Updated: 06/24/2003.
nitrite, test, nitrite, test, chemical, test, used, determine, presence, nitrite, solution, contents, chemical, methods, using, iron, sulfate, griess, test, urinary, nitrite, test, also, referenceschemical, methods, editusing, iron, sulfate, edit, simple, nitr. A nitrite test is a chemical test used to determine the presence of nitrite ion in solution Contents 1 Chemical methods 1 1 Using iron II sulfate 1 2 Griess test 2 Urinary nitrite test 3 See also 4 ReferencesChemical methods editUsing iron II sulfate edit A simple nitrite test can be performed by adding 4 M sulfuric acid to the sample until acidic and then adding 0 1 M iron II sulfate to the solution A positive test for nitrite is indicated by a dark brown solution arising from the iron nitric oxide complex ion This test is related to the brown ring test for the nitrate ion which forms the same complex in a ring In contrast nitrites turn the whole solution brown and therefore interfere with that test 1 Griess test edit Main article Griess test A common method of quantitative nitrite detection is the Griess test which relies on the reaction of nitrite with the two components of a Griess reagent to form a red azo dye This allows the concentration of nitrite to be determined by UV vis spectroscopy 2 nbsp Urinary nitrite test editA nitrite test is a standard component of a urinary test strip A positive test for nitrites in the urine is called nitrituria This test is commonly used in diagnosing urinary tract infections UTIs A positive nitrite test indicates that the cause of the UTI is a Gram negative organism most commonly Escherichia coli The reason for nitrites existence in the presence of a UTI is due to a bacterial conversion of endogenous nitrates to nitrites This may be a sign of infection However other parameters such as leukocyte esterase urine white blood cell count and symptoms such as dysuria urinary urgency fevers and chills must be correlated to diagnose an infection 3 False negative nitrite tests in urinary tract infections occur in cases with a low colony forming unit CFU count or in recently voided or dilute urine 4 In addition a nitrite test does not detect organisms unable to reduce nitrate to nitrite such as enterococci staphylococci Staphylococcus saprophyticus Acinetobacter or adenovirus 4 See also editNitrate test Saliva testing salivary nitrite levels can serve as a surrogate biomarker for nitric oxide a natural cardioprotective factor derived from nitrate rich leafy green vegetables which are often found in anti hypertensive diets such as the DASH diet 5 References edit Holltzclaw H Robinson W 1988 College Chemistry with qualitative analysis 8th ed Lexington MA D C Heath pp 1006 1007 OL 2415034M Moorcroft Matthew J Davis James Compton Richard G 2001 Detection and Determination of Nitrate and Nitrite A Review Talanta 54 5 785 803 doi 10 1016 S0039 9140 01 00323 X PMID 18968301 Blood Cell Count Control Your Health Harold Amezcua Health Testing Centers September 12 2015 a b Urinary Tract Infection Providing the Best Care By Margaret A Fitzgerald Medical Writer Sandra M Nettina Published 06 24 2002 Updated 06 24 2003 Saliva Testing Tips Friday 5 April 2019 nbsp This article about analytical chemistry is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Nitrite test amp oldid 1140501804, wikipedia, wiki, book, books, library,
