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Norbert Perrimon

December 02, 2023

Norbert Perrimon is a French geneticist and developmental biologist. He is the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School, an Investigator at the Howard Hughes Medical Institute, and an Associate of the Broad Institute. He is known for developing a number of techniques for used in genetic research with Drosophila melanogaster, as well as specific substantive contributions to signal transduction, developmental biology and physiology.

Norbert Perrimon
Born (1958-10-24) October 24, 1958 (age 65) [2]
Bosguérard-de-Marcouville[2]
NationalityFrench
CitizenshipFrance, United States
Alma materUniversity of Paris
Known forGAL4/UAS system
AwardsGeorge W. Beadle Award (2004)[1]
Scientific career
Institutions
ThesisAnalyse Clonale de Mutations en Lignee Germinale chez la Drosophile (1983)
Academic advisorsMadeleine Gans
Notable studentsSara Cherry
Website
  • perrimon.med.harvard.edu
  • www.hhmi.org/scientists/norbert-perrimon
  • fgr.hms.harvard.edu/home

Education edit

Perrimon was born in 1958 in Bosguérard-de-Marcouville, France. He earned his undergraduate degree (Maitrise of Biochemistry) at the University of Paris VI, in 1981, then completed his doctorate in 1983 with Madeleine Gans, also at the University of Paris.

Career edit

From 1983 to 1986 Perrimon was a postdoctoral researcher with Anthony Mahowald[3][4][5][6] at Case Western Reserve University, and in 1986 at the age of 27 he accepted an appointment as faculty at Harvard Medical School. He is currently the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School. He has been an Investigator of the Howard Hughes Medical Institute since 1986.[7]

Research edit

Perrimon's group developed many methods that have significantly improved the Drosophila toolbox. Perrimon co-developed the GAL4/UAS system method with Andrea Brand to control gene expression in Drosophila.[8] This method has been described as “a fly geneticist's Swiss army knife”[9] and is widely used in Drosophila genetics. Together with Tze-bin Chou, he developed the FLP-FRT DFS method to generate germline mosaics, a method that allowed the large-scale characterization of the maternal effect of zygotic lethal mutations.[10][11][12] He developed and improved methods in vivo RNAi with Janquan Ni.[13][14][15] His lab has pioneered high-throughput whole-genome RNAi screening to interrogate sysematically the function of all fly genes in various cell-based assays.[16][17][18][19][20][21][22] With Ram Viswanatha, he developed CRISPR/Cas9 pooled screens in Drosophila cells to facilitate large-scale screen in Drosophila and other arthropod cell lines.[23] The approach is particularly powerful to identify the mechanism of entry of toxins.[24]

In 2003 he created the Drosophila RNAi Screening Center at Harvard Medical School and in 2008, he initiated the Transgenic RNAi Project to generate transgenic RNAi lines for the community using optimized shRNA vectors that his lab developed.

Awards and honors edit

Perrimon was elected to the United States National Academy of Sciences in April 2013,[18][25] after naturalizing as an American citizen.

References edit

  1. ^ a b Schüpbach, T. (2004). "The 2004 George W. Beadle Medal". Genetics. 166 (2): 649–650. doi:10.1534/genetics.166.2.649. PMC 1470725. PMID 15020455.
  2. ^ a b . Archived from the original on 2014-08-08. Retrieved 2014-08-07.{{cite web}}: CS1 maint: archived copy as title (link)
  3. ^ Perrimon, N; Engstrom, L; Mahowald, A. P. (1985). "Developmental genetics of the 2C-D region of the Drosophila X chromosome". Genetics. 111 (1): 23–41. doi:10.1093/genetics/111.1.23. PMC 1202596. PMID 3928431.
  4. ^ Perrimon, N; Mohler, D; Engstrom, L; Mahowald, A. P. (1986). "X-linked female-sterile loci in Drosophila melanogaster". Genetics. 113 (3): 695–712. doi:10.1093/genetics/113.3.695. PMC 1202863. PMID 3089870.
  5. ^ Perrimon, N; Mahowald, A. P. (1986). "L(1)hopscotch, A larval-pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila". Developmental Biology. 118 (1): 28–41. doi:10.1016/0012-1606(86)90070-9. PMID 3095163.
  6. ^ Perrimon, N; Mahowald, A. P. (1987). "Multiple functions of segment polarity genes in Drosophila". Developmental Biology. 119 (2): 587–600. doi:10.1016/0012-1606(87)90061-3. PMID 3803719.
  7. ^ "Norbert Perrimon, PhD | HHMI.org". HHMI.org. Retrieved 2016-11-21.
  8. ^ Brand, A. H.; Perrimon, N. (1993). "Targeted gene expression as a means of altering cell fates and generating dominant phenotypes". Development. 118 (2): 401–415. doi:10.1242/dev.118.2.401. PMID 8223268.
  9. ^ Shetty, P. (2008). "Molecular biologist Andrea Brand: encouraging women in science". The Lancet. 371 (9617): 979. doi:10.1016/S0140-6736(08)60439-0. PMID 18358916. S2CID 33668220.
  10. ^ Chou, T. B.; Perrimon, N. (1992-07-01). "Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila". Genetics. 131 (3): 643–653. doi:10.1093/genetics/131.3.643. ISSN 0016-6731. PMC 1205036. PMID 1628809.
  11. ^ Chou, T. B.; Noll, E.; Perrimon, N. (1993-12-01). "Autosomal P[ovoD1] dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras". Development. 119 (4): 1359–1369. doi:10.1242/dev.119.4.1359. ISSN 0950-1991. PMID 8306893.
  12. ^ Chou, Tze-bin; Perrimon, Norbert (1996-12-01). "The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster". Genetics. 144 (4): 1673–1679. doi:10.1093/genetics/144.4.1673. ISSN 0016-6731. PMC 1207718. PMID 8978054.
  13. ^ Ni, Jian-Quan; Markstein, Michele; Binari, Richard; Pfeiffer, Barret; Liu, Lu-Ping; Villalta, Christians; Booker, Matthew; Perkins, Lizabeth; Perrimon, Norbert (2008-01-01). "Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster". Nature Methods. 5 (1): 49–51. doi:10.1038/nmeth1146. ISSN 1548-7091. PMC 2290002. PMID 18084299.
  14. ^ Ni, Jian-Quan; Liu, Lu-Ping; Binari, Richard; Hardy, Robert; Shim, Hye-Seok; Cavallaro, Amanda; Booker, Matthew; Pfeiffer, Barret D.; Markstein, Michele (2009-08-01). "A Drosophila resource of transgenic RNAi lines for neurogenetics". Genetics. 182 (4): 1089–1100. doi:10.1534/genetics.109.103630. ISSN 1943-2631. PMC 2728850. PMID 19487563.
  15. ^ Ni, Jian-Quan; Zhou, Rui; Czech, Benjamin; Liu, Lu-Ping; Holderbaum, Laura; Yang-Zhou, Donghui; Shim, Hye-Seok; Tao, Rong; Handler, Dominik (2011-05-01). "A genome-scale shRNA resource for transgenic RNAi in Drosophila". Nature Methods. 8 (5): 405–407. doi:10.1038/nmeth.1592. ISSN 1548-7105. PMC 3489273. PMID 21460824.
  16. ^ Boutros, Michael; Kiger, Amy A.; Armknecht, Susan; Kerr, Kim; Hild, Marc; Koch, Britta; Haas, Stefan A.; Paro, Renato; Perrimon, Norbert; Heidelberg Fly Array Consortium (2004-02-06). "Genome-wide RNAi analysis of growth and viability in Drosophila cells". Science. 303 (5659): 832–835. doi:10.1126/science.1091266. ISSN 1095-9203. PMID 14764878.
  17. ^ Perrimon, N. (2005). "Norbert Perrimon". Current Biology. 15 (13): R481–R482. doi:10.1016/j.cub.2005.06.050. PMID 16059997. S2CID 34889938.
  18. ^ a b Ravindran, S (2014). "Profile of Norbert Perrimon". Proceedings of the National Academy of Sciences. 111 (21): 7501–2. Bibcode:2014PNAS..111.7501R. doi:10.1073/pnas.1406464111. PMC 4040556. PMID 24778217.
  19. ^ Perrimon, N; Gans, M (1983). "Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237". Developmental Biology. 100 (2): 365–73. doi:10.1016/0012-1606(83)90231-2. PMID 6418585.
  20. ^ Perrimon, N (1984). "Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER". Genetics. 108 (4): 927–39. doi:10.1093/genetics/108.4.927. PMC 1224274. PMID 17246244.
  21. ^ White, R. A.; Perrimon, N; Gehring, W. J. (1984). "Differentiation markers in the Drosophila ovary". Journal of Embryology and Experimental Morphology. 84: 275–86. PMID 6442733.
  22. ^ Perrimon, N.; Engstrom, L.; Mahowald, A. P. (1984). "The effects of zygotic lethal mutations on female germ-line functions in Drosophila". Developmental Biology. 105 (2): 404–414. doi:10.1016/0012-1606(84)90297-5. PMID 6479445.
  23. ^ Viswanatha, Raghuvir; Li, Zhongchi; Hu, Yanhui; Perrimon, Norbert (2018-07-27). "Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells". eLife. 7: e36333. doi:10.7554/eLife.36333. ISSN 2050-084X. PMC 6063728. PMID 30051818.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  24. ^ Xu, Ying; Viswanatha, Raghuvir; Sitsel, Oleg; Roderer, Daniel; Zhao, Haifang; Ashwood, Christopher; Voelcker, Cecilia; Tian, Songhai; Raunser, Stefan; Perrimon, Norbert; Dong, Min (October 2022). "CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor". Nature. 610 (7931): 349–355. doi:10.1038/s41586-022-05250-7. ISSN 1476-4687. PMC 9631961. PMID 36171290.
  25. ^ "April 30, 2013, NAS Election", National Academy of Sciences (last visited May 3, 2013).
  26. ^ Teltsch, Kathleen (1985-02-10). "16 ARE GIVEN MARKEY RESEARCH SCHOLARSHIPS". The New York Times. ISSN 0362-4331. Retrieved 2023-08-28.
  27. ^ "Innovator Award Recipients". www.expressgenes.com. GeneExpression Systems. Retrieved 31 October 2016.
  28. ^ "Member Directory | American Academy of Arts and Sciences". www.amacad.org. Retrieved 2023-09-12.

December 02, 2023
norbert, perrimon, french, geneticist, developmental, biologist, james, stillman, professor, developmental, biology, department, genetics, harvard, medical, school, investigator, howard, hughes, medical, institute, associate, broad, institute, known, developin. Norbert Perrimon is a French geneticist and developmental biologist He is the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School an Investigator at the Howard Hughes Medical Institute and an Associate of the Broad Institute He is known for developing a number of techniques for used in genetic research with Drosophila melanogaster as well as specific substantive contributions to signal transduction developmental biology and physiology Norbert PerrimonBorn 1958 10 24 October 24 1958 age 65 2 Bosguerard de Marcouville 2 NationalityFrenchCitizenshipFrance United StatesAlma materUniversity of ParisKnown forGAL4 UAS systemAwardsGeorge W Beadle Award 2004 1 Scientific careerInstitutionsHarvard Medical School Howard Hughes Medical Institute Case Western Reserve UniversityThesisAnalyse Clonale de Mutations en Lignee Germinale chez la Drosophile 1983 Academic advisorsMadeleine GansNotable studentsSara CherryWebsiteperrimon wbr med wbr harvard wbr edu www wbr hhmi wbr org wbr scientists wbr norbert perrimon fgr wbr hms wbr harvard wbr edu wbr home Contents 1 Education 2 Career 2 1 Research 3 Awards and honors 4 ReferencesEducation editPerrimon was born in 1958 in Bosguerard de Marcouville France He earned his undergraduate degree Maitrise of Biochemistry at the University of Paris VI in 1981 then completed his doctorate in 1983 with Madeleine Gans also at the University of Paris Career editFrom 1983 to 1986 Perrimon was a postdoctoral researcher with Anthony Mahowald 3 4 5 6 at Case Western Reserve University and in 1986 at the age of 27 he accepted an appointment as faculty at Harvard Medical School He is currently the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School He has been an Investigator of the Howard Hughes Medical Institute since 1986 7 Research edit Perrimon s group developed many methods that have significantly improved the Drosophila toolbox Perrimon co developed the GAL4 UAS system method with Andrea Brand to control gene expression in Drosophila 8 This method has been described as a fly geneticist s Swiss army knife 9 and is widely used in Drosophila genetics Together with Tze bin Chou he developed the FLP FRT DFS method to generate germline mosaics a method that allowed the large scale characterization of the maternal effect of zygotic lethal mutations 10 11 12 He developed and improved methods in vivo RNAi with Janquan Ni 13 14 15 His lab has pioneered high throughput whole genome RNAi screening to interrogate sysematically the function of all fly genes in various cell based assays 16 17 18 19 20 21 22 With Ram Viswanatha he developed CRISPR Cas9 pooled screens in Drosophila cells to facilitate large scale screen in Drosophila and other arthropod cell lines 23 The approach is particularly powerful to identify the mechanism of entry of toxins 24 In 2003 he created the Drosophila RNAi Screening Center at Harvard Medical School and in 2008 he initiated the Transgenic RNAi Project to generate transgenic RNAi lines for the community using optimized shRNA vectors that his lab developed Awards and honors editThis section of a biography of a living person needs additional citations for verification Please help by adding reliable sources Contentious material about living persons that is unsourced or poorly sourced must be removed immediately from the article and its talk page especially if potentially libelous Find sources Norbert Perrimon news newspapers books scholar JSTOR August 2023 Learn how and when to remove this template message Perrimon was elected to the United States National Academy of Sciences in April 2013 18 25 after naturalizing as an American citizen Lucille P Markey Scholar in Biomedical Sciences 1985 26 Investigator Howard Hughes Medical Institute 1986 present Chaire d Etat College de France Paris 2003 George W Beadle Medal Genetics Society of America 2004 1 RNAi Innovator Award 2009 27 Fellow of the American Academy of Arts and Sciences 2008 28 Fellow of the American Association for the Advancement of Science 2009 Associate member of the European Molecular Biology Organization EMBO 2011 Fellow of the United States National Academy of Sciences 2013References edit a b Schupbach T 2004 The 2004 George W Beadle Medal Genetics 166 2 649 650 doi 10 1534 genetics 166 2 649 PMC 1470725 PMID 15020455 a b Archived copy Archived from the original on 2014 08 08 Retrieved 2014 08 07 a href Template Cite web html title Template Cite web cite web a CS1 maint archived copy as title link Perrimon N Engstrom L Mahowald A P 1985 Developmental genetics of the 2C D region of the Drosophila X chromosome Genetics 111 1 23 41 doi 10 1093 genetics 111 1 23 PMC 1202596 PMID 3928431 Perrimon N Mohler D Engstrom L Mahowald A P 1986 X linked female sterile loci in Drosophila melanogaster Genetics 113 3 695 712 doi 10 1093 genetics 113 3 695 PMC 1202863 PMID 3089870 Perrimon N Mahowald A P 1986 L 1 hopscotch A larval pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila Developmental Biology 118 1 28 41 doi 10 1016 0012 1606 86 90070 9 PMID 3095163 Perrimon N Mahowald A P 1987 Multiple functions of segment polarity genes in Drosophila Developmental Biology 119 2 587 600 doi 10 1016 0012 1606 87 90061 3 PMID 3803719 Norbert Perrimon PhD HHMI org HHMI org Retrieved 2016 11 21 Brand A H Perrimon N 1993 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes Development 118 2 401 415 doi 10 1242 dev 118 2 401 PMID 8223268 Shetty P 2008 Molecular biologist Andrea Brand encouraging women in science The Lancet 371 9617 979 doi 10 1016 S0140 6736 08 60439 0 PMID 18358916 S2CID 33668220 Chou T B Perrimon N 1992 07 01 Use of a yeast site specific recombinase to produce female germline chimeras in Drosophila Genetics 131 3 643 653 doi 10 1093 genetics 131 3 643 ISSN 0016 6731 PMC 1205036 PMID 1628809 Chou T B Noll E Perrimon N 1993 12 01 Autosomal P ovoD1 dominant female sterile insertions in Drosophila and their use in generating germ line chimeras Development 119 4 1359 1369 doi 10 1242 dev 119 4 1359 ISSN 0950 1991 PMID 8306893 Chou Tze bin Perrimon Norbert 1996 12 01 The Autosomal FLP DFS Technique for Generating Germline Mosaics in Drosophila melanogaster Genetics 144 4 1673 1679 doi 10 1093 genetics 144 4 1673 ISSN 0016 6731 PMC 1207718 PMID 8978054 Ni Jian Quan Markstein Michele Binari Richard Pfeiffer Barret Liu Lu Ping Villalta Christians Booker Matthew Perkins Lizabeth Perrimon Norbert 2008 01 01 Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster Nature Methods 5 1 49 51 doi 10 1038 nmeth1146 ISSN 1548 7091 PMC 2290002 PMID 18084299 Ni Jian Quan Liu Lu Ping Binari Richard Hardy Robert Shim Hye Seok Cavallaro Amanda Booker Matthew Pfeiffer Barret D Markstein Michele 2009 08 01 A Drosophila resource of transgenic RNAi lines for neurogenetics Genetics 182 4 1089 1100 doi 10 1534 genetics 109 103630 ISSN 1943 2631 PMC 2728850 PMID 19487563 Ni Jian Quan Zhou Rui Czech Benjamin Liu Lu Ping Holderbaum Laura Yang Zhou Donghui Shim Hye Seok Tao Rong Handler Dominik 2011 05 01 A genome scale shRNA resource for transgenic RNAi in Drosophila Nature Methods 8 5 405 407 doi 10 1038 nmeth 1592 ISSN 1548 7105 PMC 3489273 PMID 21460824 Boutros Michael Kiger Amy A Armknecht Susan Kerr Kim Hild Marc Koch Britta Haas Stefan A Paro Renato Perrimon Norbert Heidelberg Fly Array Consortium 2004 02 06 Genome wide RNAi analysis of growth and viability in Drosophila cells Science 303 5659 832 835 doi 10 1126 science 1091266 ISSN 1095 9203 PMID 14764878 Perrimon N 2005 Norbert Perrimon Current Biology 15 13 R481 R482 doi 10 1016 j cub 2005 06 050 PMID 16059997 S2CID 34889938 a b Ravindran S 2014 Profile of Norbert Perrimon Proceedings of the National Academy of Sciences 111 21 7501 2 Bibcode 2014PNAS 111 7501R doi 10 1073 pnas 1406464111 PMC 4040556 PMID 24778217 Perrimon N Gans M 1983 Clonal analysis of the tissue specificity of recessive female sterile mutations of Drosophila melanogaster using a dominant female sterile mutation Fs 1 K1237 Developmental Biology 100 2 365 73 doi 10 1016 0012 1606 83 90231 2 PMID 6418585 Perrimon N 1984 Clonal Analysis of Dominant Female Sterile Germline Dependent Mutations in DROSOPHILA MELANOGASTER Genetics 108 4 927 39 doi 10 1093 genetics 108 4 927 PMC 1224274 PMID 17246244 White R A Perrimon N Gehring W J 1984 Differentiation markers in the Drosophila ovary Journal of Embryology and Experimental Morphology 84 275 86 PMID 6442733 Perrimon N Engstrom L Mahowald A P 1984 The effects of zygotic lethal mutations on female germ line functions in Drosophila Developmental Biology 105 2 404 414 doi 10 1016 0012 1606 84 90297 5 PMID 6479445 Viswanatha Raghuvir Li Zhongchi Hu Yanhui Perrimon Norbert 2018 07 27 Pooled genome wide CRISPR screening for basal and context specific fitness gene essentiality in Drosophila cells eLife 7 e36333 doi 10 7554 eLife 36333 ISSN 2050 084X PMC 6063728 PMID 30051818 a href Template Cite journal html title Template Cite journal cite journal a CS1 maint unflagged free DOI link Xu Ying Viswanatha Raghuvir Sitsel Oleg Roderer Daniel Zhao Haifang Ashwood Christopher Voelcker Cecilia Tian Songhai Raunser Stefan Perrimon Norbert Dong Min October 2022 CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor Nature 610 7931 349 355 doi 10 1038 s41586 022 05250 7 ISSN 1476 4687 PMC 9631961 PMID 36171290 April 30 2013 NAS Election National Academy of Sciences last visited May 3 2013 Teltsch Kathleen 1985 02 10 16 ARE GIVEN MARKEY RESEARCH SCHOLARSHIPS The New York Times ISSN 0362 4331 Retrieved 2023 08 28 Innovator Award Recipients www expressgenes com GeneExpression Systems Retrieved 31 October 2016 Member Directory American Academy of Arts and Sciences www amacad org Retrieved 2023 09 12 Retrieved from https en wikipedia org w index php title Norbert Perrimon amp oldid 1176904267, wikipedia, wiki, book, books, library,

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