McIlvaine buffer can be used to prepare a water-soluble mounting medium when mixed 1:1 with glycerol.[2]
Contentsedit
Preparation of McIlvaine buffer requires disodium phosphate and citric acid. One liter of 0.2M stock solution of disodium hydroxyphosphate can be prepared by dissolving 28.38g of disodium phosphate in water, and adding a quantity of water sufficient to make one liter. One liter of 0.1M stock solution of citric acid can be prepared by dissolving 19.21g of citric acid in water, and adding a quantity of water sufficient to make one liter. From these stock solutions, McIlvaine buffer can be prepared in accordance with the following table:
Mixing table for obtaining 20 mL of McIlvaine buffer[1]
pH
0.2 M Na2HPO4 (mL)
0.1 M citric acid (mL)
2.2
00.40
19.60
2.4
01.24
18.76
2.6
02.18
17.82
2.8
03.17
16.83
3.0
04.11
15.89
3.2
04.94
15.06
3.4
05.70
14.30
3.6
06.44
13.56
3.8
07.10
12.90
4.0
07.71
12.29
4.2
08.28
11.72
4.4
08.82
11.18
4.6
09.35
10.65
4.8
09.86
10.14
5.0
10.30
09.70
5.2
10.72
09.28
5.4
11.15
08.85
5.6
11.60
08.40
5.8
12.09
07.91
6.0
12.63
07.37
6.2
13.22
06.78
6.4
13.85
06.15
6.6
14.55
05.45
6.8
15.45
04.55
7.0
16.47
03.53
7.2
17.39
02.61
7.4
18.17
01.83
7.6
18.73
01.27
7.8
19.15
00.85
8.0
19.45
00.55
Referencesedit
^ abMcIlvaine TC (1921). "A buffer solution for colorimetric comparison" (PDF). J. Biol. Chem. 49 (1): 183–186. doi:10.1016/S0021-9258(18)86000-8.
^"Mycoplasma Detection" (PDF). Sigma-Aldrich. Retrieved 22 January 2017.
February 09, 2024
mcilvaine, buffer, buffer, solution, composed, citric, acid, disodium, hydrogen, phosphate, also, known, citrate, phosphate, buffer, introduced, 1921, united, states, agronomist, theodore, clinton, mcilvaine, 1875, 1959, from, west, virginia, university, prepa. McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate also known as citrate phosphate buffer It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine 1875 1959 from West Virginia University and it can be prepared in pH 2 2 to 8 by mixing two stock solutions 1 Applications editMcIlvaine buffer can be used to prepare a water soluble mounting medium when mixed 1 1 with glycerol 2 Contents editPreparation of McIlvaine buffer requires disodium phosphate and citric acid One liter of 0 2M stock solution of disodium hydroxyphosphate can be prepared by dissolving 28 38g of disodium phosphate in water and adding a quantity of water sufficient to make one liter One liter of 0 1M stock solution of citric acid can be prepared by dissolving 19 21g of citric acid in water and adding a quantity of water sufficient to make one liter From these stock solutions McIlvaine buffer can be prepared in accordance with the following table Mixing table for obtaining 20 mL of McIlvaine buffer 1 pH 0 2 M Na2HPO4 mL 0 1 M citric acid mL 2 2 00 40 19 602 4 01 24 18 762 6 02 18 17 822 8 03 17 16 833 0 04 11 15 893 2 04 94 15 063 4 05 70 14 303 6 06 44 13 563 8 07 10 12 904 0 07 71 12 294 2 08 28 11 724 4 08 82 11 184 6 09 35 10 654 8 09 86 10 145 0 10 30 09 705 2 10 72 09 285 4 11 15 08 855 6 11 60 08 405 8 12 09 07 916 0 12 63 07 376 2 13 22 06 786 4 13 85 06 156 6 14 55 05 456 8 15 45 04 557 0 16 47 03 537 2 17 39 02 617 4 18 17 01 837 6 18 73 01 277 8 19 15 00 858 0 19 45 00 55References edit a b McIlvaine TC 1921 A buffer solution for colorimetric comparison PDF J Biol Chem 49 1 183 186 doi 10 1016 S0021 9258 18 86000 8 Mycoplasma Detection PDF Sigma Aldrich Retrieved 22 January 2017 Retrieved from https en wikipedia org w index php title McIlvaine buffer amp oldid 1097062667, wikipedia, wiki, book, books, library,
