This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products. It was originally called M16 medium,[1] but in 1975 Terzaghi and Sandine[2] added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many Lactobacillus species.[3]
1. Heat with frequent agitation and boil for 1 minute to completely dissolve.
2. Autoclave at 121 °C for 15 minutes. Cool to 50 °C.
3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)
Referencesedit
^Lowrie RJ, Pearce LE (1971). "The plating efficiency of bacteriophages of lactic streptococci". N.Z. J. Dairy Sci. Technol. 6: 166–171.
^ abTerzaghi BE, Sandine WE (1975). "Improved medium for lactic streptococci and their bacteriophages". Applied Microbiology. 29 (6): 807–813. doi:10.1128/am.29.6.807-813.1975. PMC187084. PMID 16350018.
^Shankar PA, Davies FL (1977). "A note on the suppression of Lactobacillus bulgaricus in media containing β-glycerophosphate and application of the media to selective isolation of Streptococcus thermophilus from yoghurt". International Journal of Dairy Technology. 30 (1): 28–30. doi:10.1111/j.1471-0307.1977.tb01162.x.
January 01, 1970
agar, this, bacterial, growth, medium, developed, 1971, lactococcus, species, isolated, from, milk, products, originally, called, medium, 1975, terzaghi, sandine, added, disodium, glycerophosphate, medium, buffer, named, growth, medium, medium, later, found, t. This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products It was originally called M16 medium 1 but in 1975 Terzaghi and Sandine 2 added disodium b glycerophosphate to the medium as a buffer and named the new growth medium M17 medium It was later found that the addition of disodium b glycerophosphate inhibits the growth of many Lactobacillus species 3 Typical composition editPer 950 mL 2 5 0 g Pancreatic digest of casein 5 0 g Soy Peptone 5 0 g Beef extract 2 5 g Yeast extract 0 5 g Ascorbic acid 0 25 g Magnesium sulfate 19 0 g Disodium b glycerophosphate 11 0 g Agar Preparation 1 Heat with frequent agitation and boil for 1 minute to completely dissolve 2 Autoclave at 121 C for 15 minutes Cool to 50 C 3 Add 50 ml filter sterilized 10 lactose solution and mix well the lactose can be exchanged to other carbohydrates e g glucose resulting in GM17 medium References edit Lowrie RJ Pearce LE 1971 The plating efficiency of bacteriophages of lactic streptococci N Z J Dairy Sci Technol 6 166 171 a b Terzaghi BE Sandine WE 1975 Improved medium for lactic streptococci and their bacteriophages Applied Microbiology 29 6 807 813 doi 10 1128 am 29 6 807 813 1975 PMC 187084 PMID 16350018 Shankar PA Davies FL 1977 A note on the suppression of Lactobacillus bulgaricus in media containing b glycerophosphate and application of the media to selective isolation of Streptococcus thermophilus from yoghurt International Journal of Dairy Technology 30 1 28 30 doi 10 1111 j 1471 0307 1977 tb01162 x Retrieved from https en wikipedia org w index php title M17 agar amp oldid 1188015407, wikipedia, wiki, book, books, library,
