LigD is a multifunctional ligase/polymerase/nuclease (3'-phosphoesterase) found in bacterial non-homologous end joining (NHEJ) DNA repair systems.[1] It is much more error-prone than the more complex eukaryotic system of NHEJ, which uses multiple enzymes to fill its role.[2] The polymerase preferentially use rNTPs (RNA nucleotides), possibly advantageous in dormant cells.[3]
Multifunctional non-homologous end joining protein LigD
The LigD homolog in Bacillus subtilis does not have the nuclease domain.
LigD with its ligase domain artificially removed can perform its function (with loss of fidelity) with a separate LigC acting as the ligase.[2]
The LigD homolog in the archaeon Methanocella paludicola is broken into three single-domain proteins sharing an operon.[4]
Referencesedit
^Della M, Palmbos PL, Tseng HM, et al. (October 2004). "Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine". Science. 306 (5696): 683–5. Bibcode:2004Sci...306..683D. doi:10.1126/science.1099824. PMID 15499016. S2CID 38823696.
^ abGong C, Bongiorno P, Martins A, et al. (April 2005). "Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity repair system driven by Ku, ligase D and ligase C". Nat. Struct. Mol. Biol. 12 (4): 304–12. doi:10.1038/nsmb915. PMID 15778718. S2CID 6879518.
^Pitcher RS, Green AJ, Brzostek A, Korycka-Machala M, Dziadek J, Doherty AJ (September 2007). "NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation" (PDF). DNA Repair (Amst.). 6 (9): 1271–6. doi:10.1016/j.dnarep.2007.02.009. PMID 17360246.
^Bartlett, EJ; Brissett, NC; Doherty, AJ (28 May 2013). "Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates". Proceedings of the National Academy of Sciences of the United States of America. 110 (22): E1984-91. Bibcode:2013PNAS..110E1984B. doi:10.1073/pnas.1302616110. PMC3670387. PMID 23671117.
January 01, 1970
ligd, multifunctional, ligase, polymerase, nuclease, phosphoesterase, found, bacterial, homologous, joining, nhej, repair, systems, much, more, error, prone, than, more, complex, eukaryotic, system, nhej, which, uses, multiple, enzymes, fill, role, polymerase,. LigD is a multifunctional ligase polymerase nuclease 3 phosphoesterase found in bacterial non homologous end joining NHEJ DNA repair systems 1 It is much more error prone than the more complex eukaryotic system of NHEJ which uses multiple enzymes to fill its role 2 The polymerase preferentially use rNTPs RNA nucleotides possibly advantageous in dormant cells 3 Multifunctional non homologous end joining protein LigDIdentifiersOrganismMycolicibacterium smegmatis ATCC 700084SymbolligDUniProtA0R3R7Search forStructuresSwiss modelDomainsInterPro The actual architecture of LigD is variable The LigD homolog in Bacillus subtilis does not have the nuclease domain LigD with its ligase domain artificially removed can perform its function with loss of fidelity with a separate LigC acting as the ligase 2 The LigD homolog in the archaeon Methanocella paludicola is broken into three single domain proteins sharing an operon 4 References edit Della M Palmbos PL Tseng HM et al October 2004 Mycobacterial Ku and ligase proteins constitute a two component NHEJ repair machine Science 306 5696 683 5 Bibcode 2004Sci 306 683D doi 10 1126 science 1099824 PMID 15499016 S2CID 38823696 a b Gong C Bongiorno P Martins A et al April 2005 Mechanism of nonhomologous end joining in mycobacteria a low fidelity repair system driven by Ku ligase D and ligase C Nat Struct Mol Biol 12 4 304 12 doi 10 1038 nsmb915 PMID 15778718 S2CID 6879518 Pitcher RS Green AJ Brzostek A Korycka Machala M Dziadek J Doherty AJ September 2007 NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation PDF DNA Repair Amst 6 9 1271 6 doi 10 1016 j dnarep 2007 02 009 PMID 17360246 Bartlett EJ Brissett NC Doherty AJ 28 May 2013 Ribonucleolytic resection is required for repair of strand displaced nonhomologous end joining intermediates Proceedings of the National Academy of Sciences of the United States of America 110 22 E1984 91 Bibcode 2013PNAS 110E1984B doi 10 1073 pnas 1302616110 PMC 3670387 PMID 23671117 Retrieved from https en wikipedia org w index php title LigD amp oldid 1129418825, wikipedia, wiki, book, books, library,
