Invasion gene associated RNA (also known as InvR) is a small non-coding RNA involved in regulating one of the major outer cell membraneporin proteins in Salmonella species.
InvR was originally predicted by computational screening the genome of Salmonella typhimurium for novel sRNA genes.[1] In this screen 46 candidate sRNA genes not conserved in Escherichia coli were identified.
The Salmonella the virulence factors that facilitate invasion of the host intestinal epithelium are located in a ~ 40 kb region in the Salmonella genome referred to as Salmonella pathogenicity Island 1 (SPI-1). The gene encoding InvR is located in this SPI-1 region between two genes called InvH and STM2901. InvR appears to be unique to Salmonella species as there does not appear to be any predicted homologues in other Enterobacteriaceae species.
InvR is ~80nt long and appears to be independently expressed from its own promoter.[1] Its expression is activated by the transcription factor HilD and has been shown to be abundantly expressed during exponential growth.[1][2] InvR has been shown to bind the RNA chaperone Hfq in vitro and Hfq is required for in vivo stability.[1] In S. typhimurium InvR RNA has been shown to repress the synthesis of the abundant outer membrane porin protein OmpD. Despite being located in the SPI-1 region there has been no link identified between the function of InvR and the SPI-1 dependent secretion pathway or invasion.
^ abcdPfeiffer V, Sittka A, Tomer R, Tedin K, Brinkmann V, Vogel J (December 2007). "A small non-coding RNA of the invasion gene island (SPI-1) represses outer membrane protein synthesis from the Salmonella core genome". Molecular Microbiology. 66 (5): 1174–1191. doi:10.1111/j.1365-2958.2007.05991.x. PMID 17971080.
^Sittka A, Lucchini S, Papenfort K, et al. (2008). Burkholder WF (ed.). "Deep sequencing analysis of small noncoding RNA and mRNA targets of the global post-transcriptional regulator, Hfq". PLOS Genetics. 4 (8): e1000163. doi:10.1371/journal.pgen.1000163. PMC2515195. PMID 18725932.
External links
Page for Invasion_gene_associated_RNA_(InvR) at Rfam
February 08, 2023
invasion, gene, associated, invr, invasion, gene, associated, also, known, invr, small, coding, involved, regulating, major, outer, cell, membrane, porin, proteins, salmonella, species, invasion, gene, associated, rnapredicted, secondary, structure, sequence, . Invasion gene associated RNA also known as InvR is a small non coding RNA involved in regulating one of the major outer cell membrane porin proteins in Salmonella species Invasion gene associated RNAPredicted secondary structure and sequence conservation of InvRIdentifiersSymbolInvRRfamRF01384Other dataRNA typeGeneDomain s BacteriaSOSO 0001263PDB structuresPDBeInvR was originally predicted by computational screening the genome of Salmonella typhimurium for novel sRNA genes 1 In this screen 46 candidate sRNA genes not conserved in Escherichia coli were identified The Salmonella the virulence factors that facilitate invasion of the host intestinal epithelium are located in a 40 kb region in the Salmonella genome referred to as Salmonella pathogenicity Island 1 SPI 1 The gene encoding InvR is located in this SPI 1 region between two genes called InvH and STM2901 InvR appears to be unique to Salmonella species as there does not appear to be any predicted homologues in other Enterobacteriaceae species InvR is 80nt long and appears to be independently expressed from its own promoter 1 Its expression is activated by the transcription factor HilD and has been shown to be abundantly expressed during exponential growth 1 2 InvR has been shown to bind the RNA chaperone Hfq in vitro and Hfq is required for in vivo stability 1 In S typhimurium InvR RNA has been shown to repress the synthesis of the abundant outer membrane porin protein OmpD Despite being located in the SPI 1 region there has been no link identified between the function of InvR and the SPI 1 dependent secretion pathway or invasion See also EditSpi 1 PU 1 5 UTR regulatory elementReferences Edit a b c d Pfeiffer V Sittka A Tomer R Tedin K Brinkmann V Vogel J December 2007 A small non coding RNA of the invasion gene island SPI 1 represses outer membrane protein synthesis from the Salmonella core genome Molecular Microbiology 66 5 1174 1191 doi 10 1111 j 1365 2958 2007 05991 x PMID 17971080 Sittka A Lucchini S Papenfort K et al 2008 Burkholder WF ed Deep sequencing analysis of small noncoding RNA and mRNA targets of the global post transcriptional regulator Hfq PLOS Genetics 4 8 e1000163 doi 10 1371 journal pgen 1000163 PMC 2515195 PMID 18725932 External links EditPage for Invasion gene associated RNA InvR at Rfam Retrieved from https en wikipedia org w index php title Invasion gene associated RNA InvR amp oldid 997749327, wikipedia, wiki, book, books, library,
