Intratracheal instillation is the introduction of a substance directly into the trachea. It is widely used to test the respiratory toxicity of a substance as an alternative to inhalation in animal testing.[1] Intratracheal instillation was reported as early as 1923 in studies of the carcinogenicity of coal tar. Modern methodology was developed by several research groups in the 1970s.[1] By contrast, tracheal administration of pharmaceutical drugs in humans is called endotracheal administration.[2]
As compared to inhalation, intratracheal instillation allows greater control over the dose and location of the substance, is cheaper and less technically demanding, allows lower amounts of scarce or expensive substances to be used, allows substances to be tested that can be inhaled by humans but not small mammals, and minimizes exposure to laboratory workers and to the skin of laboratory animals. Disadvantages include its nonphysiological and invasive nature, the confounding effects of the delivery vehicle and anesthesia, and the fact that it bypasses the upper respiratory tract. Instillation results in a less uniform distribution of the substance than inhalation, and the substance is cleared from the respiratory tract more slowly.[1] Their results provide a quick screen of potential toxicity and can be used to test its mechanism, but may not be directly applicable to occupational exposure that occurs over an extended period.[3] Some of these difficulties are overcome by another method, pharyngeal aspiration, which is less technically difficult and causes less trauma to the animal,[4] and has a pulmonary deposition pattern more similar to inhalation.[5]
Methodologyedit
Intratracheal instillation is often performed with mice, rats, or hamsters, with hamsters often preferred because their mouth can be opened widely to aid viewing the procedure,[6] and because they are more resistant to lung diseases than rats.[7] Instillation is performed either through inserting a needle or catheter down the mouth and throat, or through surgically exposing the trachea and penetrating it with a needle. Generally, short-acting inhaled anesthetic drugs such as halothane, metaphane, or enflurane are used during the instillation procedure. Saline solution is usually used as a delivery vehicle in a typical volume of 1–2 mL/kg body weight.[1] A wide range of substances can be tested, including both soluble materials and insoluble particles or fibers, including nanomaterials.[1][3][5]
^ abcdeDriscoll, Kevin E.; Costa, Daniel L.; Hatch, Gary; Henderson, Rogene; Oberdorster, Gunter; Salem, Harry; Schlesinger, Richard B. (2000-05-01). "Intratracheal Instillation as an Exposure Technique for the Evaluation of Respiratory Tract Toxicity: Uses and Limitations". Toxicological Sciences. 55 (1): 24–35. doi:10.1093/toxsci/55.1.24. ISSN 1096-6080. PMID 10788556.
^"CALS Manual: Endotracheal Drug Delivery". Comprehensive Advanced Life Support Rural Emergency Medical Education. 2011-10-13. Retrieved 2017-02-20.
^Rao, G. V. S.; Tinkle, Sally; Weissman, David; Antonini, James; Kashon, Michael; Salmen, Rebecca; Battelli, Lori; Willard, Patsy; Hubbs, Ann (2003-01-01). "Efficacy of a Technique for Exposing the Mouse Lung to Particles Aspirated from the Pharynx". Journal of Toxicology and Environmental Health, Part A. 66 (15–16): 1441–1452. doi:10.1080/15287390306417. ISSN 1528-7394. PMID 12857634. S2CID 38171132. Retrieved 2017-03-01.
^ ab"Occupational Exposure to Carbon Nanotubes and Nanofibers". U.S. National Institute for Occupational Safety and Health. April 2013. pp. 15–17, 48. Retrieved 2017-02-17.
^Zhao, Yuliang (2016-12-12). Toxicology of Nanomaterials. John Wiley & Sons. p. 163. ISBN9783527337972.
^Schiller, Erich (2013-06-29). Free Radicals and Inhalation Pathology. Springer Science & Business Media. p. 63. ISBN9783642186196.
April 10, 2024
intratracheal, instillation, introduction, substance, directly, into, trachea, widely, used, test, respiratory, toxicity, substance, alternative, inhalation, animal, testing, reported, early, 1923, studies, carcinogenicity, coal, modern, methodology, developed. Intratracheal instillation is the introduction of a substance directly into the trachea It is widely used to test the respiratory toxicity of a substance as an alternative to inhalation in animal testing 1 Intratracheal instillation was reported as early as 1923 in studies of the carcinogenicity of coal tar Modern methodology was developed by several research groups in the 1970s 1 By contrast tracheal administration of pharmaceutical drugs in humans is called endotracheal administration 2 Contents 1 Background 2 Methodology 3 See also 4 ReferencesBackground editAs compared to inhalation intratracheal instillation allows greater control over the dose and location of the substance is cheaper and less technically demanding allows lower amounts of scarce or expensive substances to be used allows substances to be tested that can be inhaled by humans but not small mammals and minimizes exposure to laboratory workers and to the skin of laboratory animals Disadvantages include its nonphysiological and invasive nature the confounding effects of the delivery vehicle and anesthesia and the fact that it bypasses the upper respiratory tract Instillation results in a less uniform distribution of the substance than inhalation and the substance is cleared from the respiratory tract more slowly 1 Their results provide a quick screen of potential toxicity and can be used to test its mechanism but may not be directly applicable to occupational exposure that occurs over an extended period 3 Some of these difficulties are overcome by another method pharyngeal aspiration which is less technically difficult and causes less trauma to the animal 4 and has a pulmonary deposition pattern more similar to inhalation 5 Methodology editIntratracheal instillation is often performed with mice rats or hamsters with hamsters often preferred because their mouth can be opened widely to aid viewing the procedure 6 and because they are more resistant to lung diseases than rats 7 Instillation is performed either through inserting a needle or catheter down the mouth and throat or through surgically exposing the trachea and penetrating it with a needle Generally short acting inhaled anesthetic drugs such as halothane metaphane or enflurane are used during the instillation procedure Saline solution is usually used as a delivery vehicle in a typical volume of 1 2 mL kg body weight 1 A wide range of substances can be tested including both soluble materials and insoluble particles or fibers including nanomaterials 1 3 5 See also editTracheal intubationReferences edit a b c d e Driscoll Kevin E Costa Daniel L Hatch Gary Henderson Rogene Oberdorster Gunter Salem Harry Schlesinger Richard B 2000 05 01 Intratracheal Instillation as an Exposure Technique for the Evaluation of Respiratory Tract Toxicity Uses and Limitations Toxicological Sciences 55 1 24 35 doi 10 1093 toxsci 55 1 24 ISSN 1096 6080 PMID 10788556 CALS Manual Endotracheal Drug Delivery Comprehensive Advanced Life Support Rural Emergency Medical Education 2011 10 13 Retrieved 2017 02 20 a b Occupational Exposure to Refractory Ceramic Fibers U S National Institute for Occupational Safety and Health May 2006 pp 38 39 42 89 Retrieved 2017 02 17 Rao G V S Tinkle Sally Weissman David Antonini James Kashon Michael Salmen Rebecca Battelli Lori Willard Patsy Hubbs Ann 2003 01 01 Efficacy of a Technique for Exposing the Mouse Lung to Particles Aspirated from the Pharynx Journal of Toxicology and Environmental Health Part A 66 15 16 1441 1452 doi 10 1080 15287390306417 ISSN 1528 7394 PMID 12857634 S2CID 38171132 Retrieved 2017 03 01 a b Occupational Exposure to Carbon Nanotubes and Nanofibers U S National Institute for Occupational Safety and Health April 2013 pp 15 17 48 Retrieved 2017 02 17 Zhao Yuliang 2016 12 12 Toxicology of Nanomaterials John Wiley amp Sons p 163 ISBN 9783527337972 Schiller Erich 2013 06 29 Free Radicals and Inhalation Pathology Springer Science amp Business Media p 63 ISBN 9783642186196 Retrieved from https en wikipedia org w index php title Intratracheal instillation amp oldid 994333578, wikipedia, wiki, book, books, library,
