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Insertion (genetics)

In genetics, an insertion (also called an insertion mutation) is the addition of one or more nucleotide base pairs into a DNA sequence. This can often happen in microsatellite regions due to the DNA polymerase slipping. Insertions can be anywhere in size from one base pair incorrectly inserted into a DNA sequence to a section of one chromosome inserted into another. The mechanism of the smallest single base insertion mutations is believed to be through base-pair separation between the template and primer strands followed by non-neighbor base stacking, which can occur locally within the DNA polymerase active site.[1] On a chromosome level, an insertion refers to the insertion of a larger sequence into a chromosome. This can happen due to unequal crossover during meiosis.

An illustration of an insertion at chromosome level

N region addition is the addition of non-coded nucleotides during recombination by terminal deoxynucleotidyl transferase.

P nucleotide insertion is the insertion of palindromic sequences encoded by the ends of the recombining gene segments.

Trinucleotide repeats are classified as insertion mutations[2][3] and sometimes as a separate class of mutations.[4]

Effects

Insertions can be particularly hazardous if they occur in an exon, the amino acid coding region of a gene. A frameshift mutation, an alteration in the normal reading frame of a gene, results if the number of inserted nucleotides is not divisible by three, i.e., the number of nucleotides per codon. Frameshift mutations will alter all the amino acids encoded by the gene following the mutation. Usually, insertions and the subsequent frameshift mutation will cause the active translation of the gene to encounter a premature stop codon, resulting in an end to translation and the production of a truncated protein. Transcripts carrying the frameshift mutation may also be degraded through Nonsense-mediated decay during translation, thus not resulting in any protein product. If translated, the truncated proteins frequently are unable to function properly or at all and can possibly result in any number of genetic disorders depending on the gene in which the insertion occurs. Methods to detect DNA sequencing errors were developed.[5]

In-frame insertions occur when the reading frame is not altered as a result of the insertion; the number of inserted nucleotides is divisible by three. The reading frame remains intact after the insertion and translation will most likely run to completion if the inserted nucleotides do not code for a stop codon. However, because of the inserted nucleotides, the finished protein will contain, depending on the size of the insertion, multiple new amino acids that may affect the function of the protein.

See also

References

  1. ^ Banavali, Nilesh K. (2013). "Partial Base Flipping is Sufficient for Strand Slippage near DNA Duplex Termini". Journal of the American Chemical Society. 135 (22): 8274–8282. doi:10.1021/ja401573j. PMID 23692220.
  2. ^ . Evolution 101: Understanding Evolution For Teachers. University of California Museum of Paleontology. Archived from the original on 2009-04-14. Retrieved 2009-09-19. ] Understanding Evolution For Teachers Home. Retrieved on September 19, 2009
  3. ^ Brown, Terence A. (2007). "16 Mutations and DNA Repair". Genomes 3. Garland Science. p. 510. ISBN 978-0-8153-4138-3.
  4. ^ Faraone, Stephen V.; Tsuang, Ming T.; Tsuang, Debby W. (1999). "5 Molecular Genetics and Mental Illness: The Search for Disease Mechanisms: Types of Mutations". Genetics of Mental Disorders: A Guide for Students, Clinicians, and Researchers. Guilford Press. p. 145. ISBN 978-1-57230-479-6.
  5. ^ Shmilovici, A.; Ben-Gal, I. (2007). "Using a VOM Model for Reconstructing Potential Coding Regions in EST Sequences" (PDF). Journal of Computational Statistics. 22 (1): 49–69. doi:10.1007/s00180-007-0021-8. S2CID 2737235.

Further reading

  • Pierce, Benjamin A. (2013). Genetics: A Conceptual Approach (5th ed.). W. H. Freeman. ISBN 978-1-4641-5084-5.

insertion, genetics, genetics, insertion, also, called, insertion, mutation, addition, more, nucleotide, base, pairs, into, sequence, this, often, happen, microsatellite, regions, polymerase, slipping, insertions, anywhere, size, from, base, pair, incorrectly,. In genetics an insertion also called an insertion mutation is the addition of one or more nucleotide base pairs into a DNA sequence This can often happen in microsatellite regions due to the DNA polymerase slipping Insertions can be anywhere in size from one base pair incorrectly inserted into a DNA sequence to a section of one chromosome inserted into another The mechanism of the smallest single base insertion mutations is believed to be through base pair separation between the template and primer strands followed by non neighbor base stacking which can occur locally within the DNA polymerase active site 1 On a chromosome level an insertion refers to the insertion of a larger sequence into a chromosome This can happen due to unequal crossover during meiosis An illustration of an insertion at chromosome level N region addition is the addition of non coded nucleotides during recombination by terminal deoxynucleotidyl transferase P nucleotide insertion is the insertion of palindromic sequences encoded by the ends of the recombining gene segments Trinucleotide repeats are classified as insertion mutations 2 3 and sometimes as a separate class of mutations 4 Contents 1 Effects 2 See also 3 References 4 Further readingEffects EditInsertions can be particularly hazardous if they occur in an exon the amino acid coding region of a gene A frameshift mutation an alteration in the normal reading frame of a gene results if the number of inserted nucleotides is not divisible by three i e the number of nucleotides per codon Frameshift mutations will alter all the amino acids encoded by the gene following the mutation Usually insertions and the subsequent frameshift mutation will cause the active translation of the gene to encounter a premature stop codon resulting in an end to translation and the production of a truncated protein Transcripts carrying the frameshift mutation may also be degraded through Nonsense mediated decay during translation thus not resulting in any protein product If translated the truncated proteins frequently are unable to function properly or at all and can possibly result in any number of genetic disorders depending on the gene in which the insertion occurs Methods to detect DNA sequencing errors were developed 5 In frame insertions occur when the reading frame is not altered as a result of the insertion the number of inserted nucleotides is divisible by three The reading frame remains intact after the insertion and translation will most likely run to completion if the inserted nucleotides do not code for a stop codon However because of the inserted nucleotides the finished protein will contain depending on the size of the insertion multiple new amino acids that may affect the function of the protein See also EditIndel Insertional mutagenesis Loss of Function Mutations Gain of Function Mutations Deletion genetics References Edit Banavali Nilesh K 2013 Partial Base Flipping is Sufficient for Strand Slippage near DNA Duplex Termini Journal of the American Chemical Society 135 22 8274 8282 doi 10 1021 ja401573j PMID 23692220 Mechanisms Genetic Variation Types of Mutations Evolution 101 Understanding Evolution For Teachers University of California Museum of Paleontology Archived from the original on 2009 04 14 Retrieved 2009 09 19 Understanding Evolution For Teachers Home Retrieved on September 19 2009 Brown Terence A 2007 16 Mutations and DNA Repair Genomes 3 Garland Science p 510 ISBN 978 0 8153 4138 3 Faraone Stephen V Tsuang Ming T Tsuang Debby W 1999 5 Molecular Genetics and Mental Illness The Search for Disease Mechanisms Types of Mutations Genetics of Mental Disorders A Guide for Students Clinicians and Researchers Guilford Press p 145 ISBN 978 1 57230 479 6 Shmilovici A Ben Gal I 2007 Using a VOM Model for Reconstructing Potential Coding Regions in EST Sequences PDF Journal of Computational Statistics 22 1 49 69 doi 10 1007 s00180 007 0021 8 S2CID 2737235 Further reading EditPierce Benjamin A 2013 Genetics A Conceptual Approach 5th ed W H Freeman ISBN 978 1 4641 5084 5 Wikimedia Commons has media related to Insertion genetics Retrieved from https en wikipedia org w index php title Insertion genetics amp oldid 1059938725, wikipedia, wiki, book, books, library,

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