Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the detection of tumor markers, such as in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours.[citation needed]
Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity. This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence of a protein combination. [1]
Referencesedit
^Young, Yoon Kow; Bolt, Alicia M.; Ahn, Ryuhjin; Mann, Koren K. (2016). "Analyzing the Tumor Microenvironment by Flow Cytometry". The Tumor Microenvironment. Methods in Molecular Biology. Vol. 1458. pp. 95–110. doi:10.1007/978-1-4939-3801-8_8. ISBN978-1-4939-3799-8. ISSN 1940-6029. PMID 27581017.
immunophenotyping, technique, used, study, protein, expressed, cells, this, technique, commonly, used, basic, science, research, laboratory, diagnostic, purpose, this, done, tissue, section, fresh, fixed, tissue, cell, suspension, example, detection, tumor, ma. Immunophenotyping is a technique used to study the protein expressed by cells This technique is commonly used in basic science research and laboratory diagnostic purpose This can be done on tissue section fresh or fixed tissue cell suspension etc An example is the detection of tumor markers such as in the diagnosis of leukemia It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane By choosing appropriate antibodies the differentiation of leukemic cells can be accurately determined The labelled cells are processed in a flow cytometer a laser based instrument capable of analyzing thousands of cells per second The whole procedure can be performed on cells from the blood bone marrow or spinal fluid in a matter of a few hours citation needed Immunophenotyping is a very common flow cytometry test in which fluorophore conjugated antibodies are used as probes for staining target cells with high avidity and affinity This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence of a protein combination 1 References edit Young Yoon Kow Bolt Alicia M Ahn Ryuhjin Mann Koren K 2016 Analyzing the Tumor Microenvironment by Flow Cytometry The Tumor Microenvironment Methods in Molecular Biology Vol 1458 pp 95 110 doi 10 1007 978 1 4939 3801 8 8 ISBN 978 1 4939 3799 8 ISSN 1940 6029 PMID 27581017 External links editBritish Society for Haematology guidelines accessed July 31 2006 Immunophenotyping at the U S National Library of Medicine Medical Subject Headings MeSH Retrieved from https en wikipedia org w index php title Immunophenotyping amp oldid 1163618660, wikipedia, wiki, book, books, library,
