Interferometric reflectance imaging sensor (IRIS), formerly known as the spectral reflectance imaging biosensor (SRIB),[1] is a system that can be used as a biosensing platform capable of high-throughput multiplexing of protein–protein, protein–DNA, and DNA–DNA interactions without the use of any fluorescent labels. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO2 substrates. IRIS is capable of quantifying biomolecular mass accumulated on the surface.[2]
To perform a measurement, the sample is illuminated with multiple different wavelengths from either a tunable laser or different color LEDs; typically speaking, a relatively narrow bandwidth optical source. The reflection intensity is imaged using a CCD or CMOS camera. By using interferometric techniques, nanometer changes can be detected.
Applicationsedit
Applications for IRIS include microarray format immunoassays, single nucleotide polymorphism (SNP)[3] detection, pathogen detection and bio-defense monitoring, kinetic analysis of biomolecular interactions, and general biomolecular interaction studies for research applications.
Referencesedit
^ E. Ozkumur; et al. (2008). "Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications". Proceedings of the National Academy of Sciences. 105 (23): 7988–7992. Bibcode:2008PNAS..105.7988O. doi:10.1073/pnas.0711421105. PMC2430348. PMID 18523019.
^E. Ozkumur; et al. (2009). "Quantification of DNA and protein adsorption by optical phase shift". Biosensors and Bioelectronics. 25 (1): 167–172. doi:10.1016/j.bios.2009.06.033. PMC2750076. PMID 19628383.
^E. Ozkumur; et al. (2010). "Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches". Biosensors and Bioelectronics. 25 (7): 1789–1795. doi:10.1016/j.bios.2009.12.032. PMC2824047. PMID 20097056.
External linksedit
January 01, 1970
iris, biosensor, interferometric, reflectance, imaging, sensor, iris, formerly, known, spectral, reflectance, imaging, biosensor, srib, system, that, used, biosensing, platform, capable, high, throughput, multiplexing, protein, protein, protein, interactions, . Interferometric reflectance imaging sensor IRIS formerly known as the spectral reflectance imaging biosensor SRIB 1 is a system that can be used as a biosensing platform capable of high throughput multiplexing of protein protein protein DNA and DNA DNA interactions without the use of any fluorescent labels The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si SiO2 substrates IRIS is capable of quantifying biomolecular mass accumulated on the surface 2 Contents 1 Measurement 2 Applications 3 References 4 External linksMeasurement editTo perform a measurement the sample is illuminated with multiple different wavelengths from either a tunable laser or different color LEDs typically speaking a relatively narrow bandwidth optical source The reflection intensity is imaged using a CCD or CMOS camera By using interferometric techniques nanometer changes can be detected Applications editApplications for IRIS include microarray format immunoassays single nucleotide polymorphism SNP 3 detection pathogen detection and bio defense monitoring kinetic analysis of biomolecular interactions and general biomolecular interaction studies for research applications References edit E Ozkumur et al 2008 Label free and dynamic detection of biomolecular interactions for high throughput microarray applications Proceedings of the National Academy of Sciences 105 23 7988 7992 Bibcode 2008PNAS 105 7988O doi 10 1073 pnas 0711421105 PMC 2430348 PMID 18523019 E Ozkumur et al 2009 Quantification of DNA and protein adsorption by optical phase shift Biosensors and Bioelectronics 25 1 167 172 doi 10 1016 j bios 2009 06 033 PMC 2750076 PMID 19628383 E Ozkumur et al 2010 Label free microarray imaging for direct detection of DNA hybridization and single nucleotide mismatches Biosensors and Bioelectronics 25 7 1789 1795 doi 10 1016 j bios 2009 12 032 PMC 2824047 PMID 20097056 External links editIRIS homepage Retrieved from https en wikipedia org w index php title IRIS biosensor amp oldid 1042055151, wikipedia, wiki, book, books, library,
