The Pseudomonas syringaeHrpZ Cation Channel (HrpZ) Family (TC# 1.C.56) is a member of the RTX-toxin superfamily. The Harpin-PSS (HrpZ; TC# 1.C.56.1.1) protein is secreted by Pseudomonas syringae via the Hrp secretion system (IIISP; TC# 3.A.6) and elicits a hypersensitive response (HR) in non-host plants upon infection and pathogenicity in hosts.[1] It contains several repetitive regions and exhibits two extended (20 residue) regions of moderate hydrophobicity that might serve as α-helical TMSs. The HrpZ cation channel is predicted to be largely of α-structure. HrpZ - a harpin - is a highly thermostable protein that exhibits multifunctional abilities, e.g., it elicits the hypersensitive response (HR), enhances plant growth, acts as a virulence factor, and forms pores in plant plasma membranes as well as artificial membranes.[2] Homologues are not found in organisms other than P. syringae.[3][4]
When inserted into liposomes and synthetic bilayers at low concentrations (2 nM), it provokes a cation-selective ion current with large unitary conductance. Chloride is not transported. It has been hypothesized that such channels could allow nutrient release and/or delivery of virulence factors during bacterial colonization of host plants. The leucine-zipper-like motifs may take part in the formation of oligomeric aggregates, and oligomerization could be related to HR elicitation.[3]
Transport reactionedit
The generalized transport reaction thought to be catalyzed by HrpZ is:[4]
Small molecules (in) → Small molecules (out)
Referencesedit
^Haapalainen, Minna; Engelhardt, Stefan; Küfner, Isabell; Li, Chun-Mei; Nürnberger, Thorsten; Lee, Justin; Romantschuk, Martin; Taira, Suvi (2011-02-01). "Functional mapping of harpin HrpZ of Pseudomonas syringae reveals the sites responsible for protein oligomerization, lipid interactions and plant defence induction". Molecular Plant Pathology. 12 (2): 151–166. doi:10.1111/j.1364-3703.2010.00655.x. ISSN 1364-3703. PMC6640321. PMID 21199565.
^ abLee, J.; Klusener, B.; Tsiamis, G.; Stevens, C.; Neyt, C.; Tampakaki, A. P.; Panopoulos, N. J.; Nöller, J.; Weiler, E. W. (2001-01-02). "HrpZ(Psph) from the plant pathogen Pseudomonas syringae pv. phaseolicola binds to lipid bilayers and forms an ion-conducting pore in vitro". Proceedings of the National Academy of Sciences of the United States of America. 98 (1): 289–294. doi:10.1073/pnas.011265298. ISSN 0027-8424. PMC14583. PMID 11134504.
^ abSaier, MH Jr. "1.C.56 The Pseudomonas syringae HrpZ Target Host Cell Membrane Cation Channel (HrpZ) Family". Transporter Classification Database. Saier Lab Bioinformatics Group / SDSC.
Further readingedit
Chen, Jonathan S.; Reddy, Vamsee; Chen, Joshua H.; Shlykov, Maksim A.; Zheng, Wei Hao; Cho, Jaehoon; Yen, Ming Ren; Saier, Milton H. (2011). "Phylogenetic characterization of transport protein superfamilies: superiority of SuperfamilyTree programs over those based on multiple alignments". Journal of Molecular Microbiology and Biotechnology. 21 (3–4): 83–96. doi:10.1159/000334611. PMC3290041. PMID 22286036.
April 12, 2024
hrpz, family, pseudomonas, syringae, hrpz, cation, channel, hrpz, family, member, toxin, superfamily, harpin, hrpz, protein, secreted, pseudomonas, syringae, secretion, system, iiisp, elicits, hypersensitive, response, host, plants, upon, infection, pathogenic. The Pseudomonas syringae HrpZ Cation Channel HrpZ Family TC 1 C 56 is a member of the RTX toxin superfamily The Harpin PSS HrpZ TC 1 C 56 1 1 protein is secreted by Pseudomonas syringae via the Hrp secretion system IIISP TC 3 A 6 and elicits a hypersensitive response HR in non host plants upon infection and pathogenicity in hosts 1 It contains several repetitive regions and exhibits two extended 20 residue regions of moderate hydrophobicity that might serve as a helical TMSs The HrpZ cation channel is predicted to be largely of a structure HrpZ a harpin is a highly thermostable protein that exhibits multifunctional abilities e g it elicits the hypersensitive response HR enhances plant growth acts as a virulence factor and forms pores in plant plasma membranes as well as artificial membranes 2 Homologues are not found in organisms other than P syringae 3 4 Contents 1 Function 1 1 Transport reaction 2 References 3 Further readingFunction editWhen inserted into liposomes and synthetic bilayers at low concentrations 2 nM it provokes a cation selective ion current with large unitary conductance Chloride is not transported It has been hypothesized that such channels could allow nutrient release and or delivery of virulence factors during bacterial colonization of host plants The leucine zipper like motifs may take part in the formation of oligomeric aggregates and oligomerization could be related to HR elicitation 3 Transport reaction edit The generalized transport reaction thought to be catalyzed by HrpZ is 4 Small molecules in Small molecules out References edit Haapalainen Minna Engelhardt Stefan Kufner Isabell Li Chun Mei Nurnberger Thorsten Lee Justin Romantschuk Martin Taira Suvi 2011 02 01 Functional mapping of harpin HrpZ of Pseudomonas syringae reveals the sites responsible for protein oligomerization lipid interactions and plant defence induction Molecular Plant Pathology 12 2 151 166 doi 10 1111 j 1364 3703 2010 00655 x ISSN 1364 3703 PMC 6640321 PMID 21199565 Choi Min Seon Kim Wooki Lee Chanhui Oh Chang Sik 2013 10 01 Harpins multifunctional proteins secreted by gram negative plant pathogenic bacteria Molecular Plant Microbe Interactions 26 10 1115 1122 doi 10 1094 MPMI 02 13 0050 CR ISSN 0894 0282 PMID 23745678 a b Lee J Klusener B Tsiamis G Stevens C Neyt C Tampakaki A P Panopoulos N J Noller J Weiler E W 2001 01 02 HrpZ Psph from the plant pathogen Pseudomonas syringae pv phaseolicola binds to lipid bilayers and forms an ion conducting pore in vitro Proceedings of the National Academy of Sciences of the United States of America 98 1 289 294 doi 10 1073 pnas 011265298 ISSN 0027 8424 PMC 14583 PMID 11134504 a b Saier MH Jr 1 C 56 The Pseudomonas syringae HrpZ Target Host Cell Membrane Cation Channel HrpZ Family Transporter Classification Database Saier Lab Bioinformatics Group SDSC Further reading editChen Jonathan S Reddy Vamsee Chen Joshua H Shlykov Maksim A Zheng Wei Hao Cho Jaehoon Yen Ming Ren Saier Milton H 2011 Phylogenetic characterization of transport protein superfamilies superiority of SuperfamilyTree programs over those based on multiple alignments Journal of Molecular Microbiology and Biotechnology 21 3 4 83 96 doi 10 1159 000334611 PMC 3290041 PMID 22286036 Retrieved from https en wikipedia org w index php title HrpZ Family amp oldid 1109234528, wikipedia, wiki, book, books, library,
