H295R (also referred to as NCI-H295R) is an angiotensin-II-responsive steroid-producing adrenocorticalcell line.[1] It was initially isolated in 1980 from a 48-year-old female patient diagnosed with adrenocortical carcinoma.[1][2] The initial polyclonal populations of tumor cells obtained from the patients' tumor were cultured and the resultant cell line was called NCI-H295.[1][2] Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth.[1] Three strains were developed, based on the serum supplement used for growth, which have been termed H295R-S1, H295R-S2 and H295R-S3.[1][3] All three strains grow as adherent monolayer cultures.[1]
^ abGazdar AF, Oie HK, Shackleton CH, Chen TR, Triche TJ, Myers CE, Chrousos GP, Brennan MF, Stein CA, La Rocca RV (1990). "Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis". Cancer Res. 50 (17): 5488–5496. PMID 2386954.
h295r, also, referred, angiotensin, responsive, steroid, producing, adrenocortical, cell, line, initially, isolated, 1980, from, year, female, patient, diagnosed, with, adrenocortical, carcinoma, initial, polyclonal, populations, tumor, cells, obtained, from, . H295R also referred to as NCI H295R is an angiotensin II responsive steroid producing adrenocortical cell line 1 It was initially isolated in 1980 from a 48 year old female patient diagnosed with adrenocortical carcinoma 1 2 The initial polyclonal populations of tumor cells obtained from the patients tumor were cultured and the resultant cell line was called NCI H295 1 2 Because of slow growth rates and easy detachment of the original NCI H295 strains efforts were made to select a population of cells with better monolayer attachment and more rapid growth 1 Three strains were developed based on the serum supplement used for growth which have been termed H295R S1 H295R S2 and H295R S3 1 3 All three strains grow as adherent monolayer cultures 1 References edit a b c d e f Wang T Rainey WE 2012 Human Adrenocortical Carcinoma Cell Lines Mol Cell Endocrinol 351 1 58 65 doi 10 1016 j mce 2011 08 041 PMC 3288152 PMID 21924324 a b Gazdar AF Oie HK Shackleton CH Chen TR Triche TJ Myers CE Chrousos GP Brennan MF Stein CA La Rocca RV 1990 Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis Cancer Res 50 17 5488 5496 PMID 2386954 Rainey WE Saner K Schimmer BP 2004 Adrenocortical cell lines Mol Cell Endocrinol 228 1 2 23 38 doi 10 1016 j mce 2003 12 020 PMID 15541570 S2CID 1628345 External links editCellosaurus entry for H295R Retrieved from https en wikipedia org w index php title H295R amp oldid 1161653340, wikipedia, wiki, book, books, library,
