An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell.[1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.[2]
An expression cassette is composed of one or more genes and the sequences controlling their expression.[3] An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.[4]
Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.[5][6]
^Papadakis, E.D.; et al. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy. 4 (4): 89–113. CiteSeerX10.1.1.494.3472. doi:10.2174/1566523044578077. PMID 15032617.
^Bertrand, Edouard; et al. (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization". RNA. 3 (1): 75–88. PMC1369464. PMID 8990401.
^Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories. 12 (96): 96. doi:10.1186/1475-2859-12-96. PMC4231455. PMID 24161108.
^Gray, Steven James; et al. (January 2015). "Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy". Discov Med. 19 (102): 49–57. ISSN 1539-6509. PMC4505817. PMID 25636961.
^Azhakanandam, K.; et al. (2015). "Plant Trait Gene Expression Cassette Design". Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer. pp. 41–52. doi:10.1007/978-1-4939-2202-4_2. ISBN978-1-4939-2201-7. S2CID 82188623.
^Chow, Yu-Hua; et al. (December 1997). "Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways" (PDF). Proceedings of the National Academy of Sciences. 94 (26): 14695–14700. Bibcode:1997PNAS...9414695C. doi:10.1073/pnas.94.26.14695. PMC25096. PMID 9405675.
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expression, cassette, expression, cassette, distinct, component, vector, consisting, gene, regulatory, sequence, expressed, transfected, cell, each, successful, transformation, expression, cassette, directs, cell, machinery, make, protein, some, expression, ca. An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell 1 In each successful transformation the expression cassette directs the cell s machinery to make RNA and protein s Some expression cassettes are designed for modular cloning of protein encoding sequences so that the same cassette can easily be altered to make different proteins 2 An expression cassette is composed of one or more genes and the sequences controlling their expression 3 An expression cassette comprises three components a promoter sequence an open reading frame and a 3 untranslated region that in eukaryotes usually contains a polyadenylation site 4 Different expression cassettes can be transfected into different organisms including bacteria yeast plants and mammalian cells as long as the correct regulatory sequences are used 5 6 See also editExpression vector Gene cassetteReferences edit Papadakis E D et al 2004 Promoters and Control Elements Designing Expression Cassettes for Gene Therapy Current Gene Therapy 4 4 89 113 CiteSeerX 10 1 1 494 3472 doi 10 2174 1566523044578077 PMID 15032617 Bertrand Edouard et al 1997 The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization RNA 3 1 75 88 PMC 1369464 PMID 8990401 Vickers Claudia E December 2013 Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae Microbial Cell Factories 12 96 96 doi 10 1186 1475 2859 12 96 PMC 4231455 PMID 24161108 Gray Steven James et al January 2015 Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy Discov Med 19 102 49 57 ISSN 1539 6509 PMC 4505817 PMID 25636961 Azhakanandam K et al 2015 Plant Trait Gene Expression Cassette Design Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants Springer pp 41 52 doi 10 1007 978 1 4939 2202 4 2 ISBN 978 1 4939 2201 7 S2CID 82188623 Chow Yu Hua et al December 1997 Development of an epithelium specific expression cassette with human DNA regulatory elements for transgene expression in lung airways PDF Proceedings of the National Academy of Sciences 94 26 14695 14700 Bibcode 1997PNAS 9414695C doi 10 1073 pnas 94 26 14695 PMC 25096 PMID 9405675 nbsp This biochemistry article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Expression cassette amp oldid 1188209365, wikipedia, wiki, book, books, library,
