Encapsulin nanocompartments, or encapsulin protein cages, are spherical bacterial organelle-like compartments roughly 25-30 nm in diameter that are involved in various aspects of metabolism, in particular protecting bacteria from oxidative stress. Encapsulin nanocompartments are structurally similar to the HK97 bacteriophage and their function depends on the proteins loaded into the nanocompartment.[1] The sphere is formed from 60 (for a 25 nm sphere) or 180 (for a 30 nm sphere) copies of a single protomer, termed encapsulin. Their structure has been studied in great detail using X-ray crystallography[2] and cryo-electron microscopy.[3]
A number of different types of proteins have been identified as being loaded into encapsulin nanocompartments. Peroxidases or proteins similar to ferritins are the two most common types of cargo proteins.[4] While most encapsulin nanocompartments contain only one type of cargo protein, in some species two or three types of cargo proteins are loaded.[2][3][4]
Encapsulins purified from Rhodococcus jostii can be assembled and disassembled with changes in pH. In the assembled state, the compartment enhances the activity of its cargo, a peroxidase enzyme.[5]
Use as a platform for bioengineeringedit
Recently, encapsulin nanocompartments have begun to receive considerable interest from bioengineers because of their potential to allow the targeted delivery of drugs, proteins, and mRNAs to specific cells of interest.[6][7][8]
Referencesedit
^Nichols, Robert J.; Cassidy-Amstutz, Caleb; Chaijarasphong, Thawatchai; Savage, David F. (October 2017). "Encapsulins: molecular biology of the shell". Critical Reviews in Biochemistry and Molecular Biology. 52 (5): 583–594. doi:10.1080/10409238.2017.1337709. ISSN 1549-7798. PMID 28635326. S2CID 205695184.
^ abSutter, M; Boehringer, D; Gutmann, S; Günther, S; Prangishvili, D; Loessner, MJ; Stetter, KO; Weber-Ban, E; Ban, N (September 2008). "Structural basis of enzyme encapsulation into a bacterial nanocompartment". Nature Structural & Molecular Biology. 15 (9): 939–47. doi:10.1038/nsmb.1473. hdl:20.500.11850/150838. PMID 19172747. S2CID 205522743.
^ abMcHugh, CA; Fontana, J; Nemecek, D; Cheng, N; Aksyuk, AA; Heymann, JB; Winkler, DC; Lam, AS; Wall, JS; Steven, AC; Hoiczyk, E (1 September 2014). "A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress". The EMBO Journal. 33 (17): 1896–911. doi:10.15252/embj.201488566. PMC4195785. PMID 25024436.
^ abGiessen, Tobias W.; Silver, Pamela A. (2017-03-06). "Widespread distribution of encapsulin nanocompartments reveals functional diversity". Nature Microbiology. 2 (6): 17029. doi:10.1038/nmicrobiol.2017.29. ISSN 2058-5276. PMID 28263314. S2CID 5549808.
^Rahmanpour, Rahman; Bugg, Timothy D. H. (2013-05-01). "Assembly in vitro of Rhodococcus jostii RHA1 encapsulin and peroxidase DypB to form a nanocompartment" (PDF). The FEBS Journal. 280 (9): 2097–2104. doi:10.1111/febs.12234. ISSN 1742-4658. PMID 23560779.
^Corchero, José L; Cedano, Juan (2011). "Self-assembling, protein-based intracellular bacterial organelles: emerging vehicles for encapsulating, targeting and delivering therapeutical cargoes". Microbial Cell Factories. 10 (1): 92. doi:10.1186/1475-2859-10-92. PMC3247854. PMID 22046962.
^Moon, H; Lee, J; Kim, H; Heo, S; Min, J; Kang, S (2014). "Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe". Biomaterials Research. 18: 21. doi:10.1186/2055-7124-18-21. PMC4552281. PMID 26331071.
^Tamura, A; Fukutani, Y; Takami, T; Fujii, M; Nakaguchi, Y; Murakami, Y; Noguchi, K; Yohda, M; Odaka, M (January 2015). "Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771". Biotechnology and Bioengineering. 112 (1): 13–20. doi:10.1002/bit.25322. PMID 24981030. S2CID 205504195.
January 01, 1970
encapsulin, nanocompartment, encapsulin, protein, cages, spherical, bacterial, organelle, like, compartments, roughly, diameter, that, involved, various, aspects, metabolism, particular, protecting, bacteria, from, oxidative, stress, structurally, similar, hk9. Encapsulin nanocompartments or encapsulin protein cages are spherical bacterial organelle like compartments roughly 25 30 nm in diameter that are involved in various aspects of metabolism in particular protecting bacteria from oxidative stress Encapsulin nanocompartments are structurally similar to the HK97 bacteriophage and their function depends on the proteins loaded into the nanocompartment 1 The sphere is formed from 60 for a 25 nm sphere or 180 for a 30 nm sphere copies of a single protomer termed encapsulin Their structure has been studied in great detail using X ray crystallography 2 and cryo electron microscopy 3 Crystal structure of Thermotoga maritima encapsulin PDB entry 3dkt A number of different types of proteins have been identified as being loaded into encapsulin nanocompartments Peroxidases or proteins similar to ferritins are the two most common types of cargo proteins 4 While most encapsulin nanocompartments contain only one type of cargo protein in some species two or three types of cargo proteins are loaded 2 3 4 Encapsulins purified from Rhodococcus jostii can be assembled and disassembled with changes in pH In the assembled state the compartment enhances the activity of its cargo a peroxidase enzyme 5 Use as a platform for bioengineering editRecently encapsulin nanocompartments have begun to receive considerable interest from bioengineers because of their potential to allow the targeted delivery of drugs proteins and mRNAs to specific cells of interest 6 7 8 References edit Nichols Robert J Cassidy Amstutz Caleb Chaijarasphong Thawatchai Savage David F October 2017 Encapsulins molecular biology of the shell Critical Reviews in Biochemistry and Molecular Biology 52 5 583 594 doi 10 1080 10409238 2017 1337709 ISSN 1549 7798 PMID 28635326 S2CID 205695184 a b Sutter M Boehringer D Gutmann S Gunther S Prangishvili D Loessner MJ Stetter KO Weber Ban E Ban N September 2008 Structural basis of enzyme encapsulation into a bacterial nanocompartment Nature Structural amp Molecular Biology 15 9 939 47 doi 10 1038 nsmb 1473 hdl 20 500 11850 150838 PMID 19172747 S2CID 205522743 a b McHugh CA Fontana J Nemecek D Cheng N Aksyuk AA Heymann JB Winkler DC Lam AS Wall JS Steven AC Hoiczyk E 1 September 2014 A virus capsid like nanocompartment that stores iron and protects bacteria from oxidative stress The EMBO Journal 33 17 1896 911 doi 10 15252 embj 201488566 PMC 4195785 PMID 25024436 a b Giessen Tobias W Silver Pamela A 2017 03 06 Widespread distribution of encapsulin nanocompartments reveals functional diversity Nature Microbiology 2 6 17029 doi 10 1038 nmicrobiol 2017 29 ISSN 2058 5276 PMID 28263314 S2CID 5549808 Rahmanpour Rahman Bugg Timothy D H 2013 05 01 Assembly in vitro of Rhodococcus jostii RHA1 encapsulin and peroxidase DypB to form a nanocompartment PDF The FEBS Journal 280 9 2097 2104 doi 10 1111 febs 12234 ISSN 1742 4658 PMID 23560779 Corchero Jose L Cedano Juan 2011 Self assembling protein based intracellular bacterial organelles emerging vehicles for encapsulating targeting and delivering therapeutical cargoes Microbial Cell Factories 10 1 92 doi 10 1186 1475 2859 10 92 PMC 3247854 PMID 22046962 Moon H Lee J Kim H Heo S Min J Kang S 2014 Genetically engineering encapsulin protein cage nanoparticle as a SCC 7 cell targeting optical nanoprobe Biomaterials Research 18 21 doi 10 1186 2055 7124 18 21 PMC 4552281 PMID 26331071 Tamura A Fukutani Y Takami T Fujii M Nakaguchi Y Murakami Y Noguchi K Yohda M Odaka M January 2015 Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771 Biotechnology and Bioengineering 112 1 13 20 doi 10 1002 bit 25322 PMID 24981030 S2CID 205504195 Retrieved from https en wikipedia org w index php title Encapsulin nanocompartment amp oldid 1219972453, wikipedia, wiki, book, books, library,
