A DNA field-effect transistor (DNAFET) is a field-effect transistor which uses the field-effect due to the partial charges of DNA molecules to function as a biosensor. The structure of DNAFETs is similar to that of MOSFETs, with the exception of the gate structure which, in DNAFETs, is replaced by a layer of immobilized ssDNA (single-stranded DNA) molecules which act as surface receptors. When complementary DNA strands hybridize to the receptors, the charge distribution near the surface changes, which in turn modulates current transport through the semiconductor transducer.
Arrays of DNAFETs can be used for detecting single nucleotide polymorphisms (causing many hereditary diseases) and for DNA sequencing. Their main advantage compared to optical detection methods in common use today is that they do not require labeling of molecules. Furthermore, they work continuously and (near) real-time. DNAFETs are highly selective since only specific binding modulates charge transport.
Referencesedit
Li Z, Chen Y, Li X, Kamins TI, Nauka K, Williams RS (2004). "Sequence-Specific Label-Free DNA Sensors Based on Silicon Nanowires". Nano Lett. 4 (2): 245–7. Bibcode:2004NanoL...4..245L. doi:10.1021/nl034958e.
Souteyrand E, Cloarec JP, Martin JR, Wilson C, Lawrence I, Mikkelsen S, Lawrence MF (1997). "Direct Detection of the Hybridization of Synthetic Homo-Oligomer DNA Sequences by Field Effect". J. Phys. Chem. B. 101 (15): 2980–5. doi:10.1021/jp963056h.
Fritz J, Cooper EB, Gaudet S, Sorger PK, Manalis SR (October 2002). "Electronic detection of DNA by its intrinsic molecular charge". Proc. Natl. Acad. Sci. U.S.A. 99 (22): 14142–6. Bibcode:2002PNAS...9914142F. doi:10.1073/pnas.232276699. PMC137851. PMID 12386345.
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field, effect, transistor, dnafet, field, effect, transistor, which, uses, field, effect, partial, charges, molecules, function, biosensor, structure, dnafets, similar, that, mosfets, with, exception, gate, structure, which, dnafets, replaced, layer, immobiliz. A DNA field effect transistor DNAFET is a field effect transistor which uses the field effect due to the partial charges of DNA molecules to function as a biosensor The structure of DNAFETs is similar to that of MOSFETs with the exception of the gate structure which in DNAFETs is replaced by a layer of immobilized ssDNA single stranded DNA molecules which act as surface receptors When complementary DNA strands hybridize to the receptors the charge distribution near the surface changes which in turn modulates current transport through the semiconductor transducer Arrays of DNAFETs can be used for detecting single nucleotide polymorphisms causing many hereditary diseases and for DNA sequencing Their main advantage compared to optical detection methods in common use today is that they do not require labeling of molecules Furthermore they work continuously and near real time DNAFETs are highly selective since only specific binding modulates charge transport References editLi Z Chen Y Li X Kamins TI Nauka K Williams RS 2004 Sequence Specific Label Free DNA Sensors Based on Silicon Nanowires Nano Lett 4 2 245 7 Bibcode 2004NanoL 4 245L doi 10 1021 nl034958e Souteyrand E Cloarec JP Martin JR Wilson C Lawrence I Mikkelsen S Lawrence MF 1997 Direct Detection of the Hybridization of Synthetic Homo Oligomer DNA Sequences by Field Effect J Phys Chem B 101 15 2980 5 doi 10 1021 jp963056h Fritz J Cooper EB Gaudet S Sorger PK Manalis SR October 2002 Electronic detection of DNA by its intrinsic molecular charge Proc Natl Acad Sci U S A 99 22 14142 6 Bibcode 2002PNAS 9914142F doi 10 1073 pnas 232276699 PMC 137851 PMID 12386345 nbsp nbsp This bioinformatics related article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title DNA field effect transistor amp oldid 1107601793, wikipedia, wiki, book, books, library,
