A cellular microarray (or cell microarray) is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support. The support, sometimes called a "chip", is spotted with varying materials, such as antibodies, proteins, or lipids, which can interact with the cells, leading to their capture on specific spots. Combinations of different materials can be spotted in a given area, allowing not only cellular capture, when a specific interaction exists, but also the triggering of a cellular response, change in phenotype, or detection of a response from the cell, such as a specific secreted factor.
There are a large number of types of cellular microarrays:
Reverse transfection cell microarrays. David M. Sabatini's laboratory developed reverse-transfection cell microarrays at the Whitehead Institute, publishing their work in 2001.
Chen DS, Davis MM (2006) Molecular and functional analysis using live cell microarrays. Curr Opin Chem Biol 10:28-34
Chen DS, Soen Y, Stuge TB, Lee PP, Weber JS, Brown PO, Davis MM (2005) Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray. PLoS Med 2: 10: e265 ([1])
Chen DS, Davis MM (2005) Cellular immunotherapy: Antigen recognition is just the beginning. Springer Semin Immunopathol 27:119–127
Chen DS, Soen Y, Davis MM, Brown PO (2004) Functional and molecular profiling of heterogeneous tumor samples using a novel cellular microarray. J Clin Oncol 22:9507 ()
Soen Y, Chen DS, Stuge TB, Weber JS, Lee PP, et al. (2004) A novel cellular microarray identifies functional deficiences in tumor-specific T cell responses. J Clin Oncol 22:2510
Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature. 2001 May 3;411(6833):107-10. Pubmed link
April 27, 2024
cellular, microarray, this, article, includes, list, references, related, reading, external, links, sources, remain, unclear, because, lacks, inline, citations, please, help, improve, this, article, introducing, more, precise, citations, september, 2019, learn. This article includes a list of references related reading or external links but its sources remain unclear because it lacks inline citations Please help improve this article by introducing more precise citations September 2019 Learn how and when to remove this template message A cellular microarray or cell microarray is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support The support sometimes called a chip is spotted with varying materials such as antibodies proteins or lipids which can interact with the cells leading to their capture on specific spots Combinations of different materials can be spotted in a given area allowing not only cellular capture when a specific interaction exists but also the triggering of a cellular response change in phenotype or detection of a response from the cell such as a specific secreted factor There are a large number of types of cellular microarrays Reverse transfection cell microarrays David M Sabatini s laboratory developed reverse transfection cell microarrays at the Whitehead Institute publishing their work in 2001 PMHC Cellular Microarrays This type of microarray were developed by Daniel Chen Yoav Soen Dan Kraft Patrick Brown and Mark Davis at Stanford University Medical Center References editChen DS Davis MM 2006 Molecular and functional analysis using live cell microarrays Curr Opin Chem Biol 10 28 34 Chen DS Soen Y Stuge TB Lee PP Weber JS Brown PO Davis MM 2005 Marked Differences in Human Melanoma Antigen Specific T Cell Responsiveness after Vaccination Using a Functional Microarray PLoS Med 2 10 e265 1 nbsp Soen Y Chen D S Kraft D L Davis M M and Brown P O 2003 Detection and characterization of cellular immune responses using peptide MHC microarrays PLoS Biol 1 E65 http biology plosjournals org perlserv request get document amp doi 10 1371 journal pbio 0000065 nbsp Chen DS Davis MM 2005 Cellular immunotherapy Antigen recognition is just the beginning Springer Semin Immunopathol 27 119 127 Chen DS Soen Y Davis MM Brown PO 2004 Functional and molecular profiling of heterogeneous tumor samples using a novel cellular microarray J Clin Oncol 22 9507 https web archive org web 20041020122342 http meeting jco org cgi content abstract 22 14 suppl 9507 Soen Y Chen DS Stuge TB Weber JS Lee PP et al 2004 A novel cellular microarray identifies functional deficiences in tumor specific T cell responses J Clin Oncol 22 2510 Ziauddin J Sabatini DM 2001 Microarrays of cells expressing defined cDNAs Nature 2001 May 3 411 6833 107 10 Pubmed link Retrieved from https en wikipedia org w index php title Cellular microarray amp oldid 972348100, wikipedia, wiki, book, books, library,
