Unlike eukaryotic mRNA, this virus lacks a 5' cap and a poly(A) tail but still circularises its mRNA through base pairing between two stem loops, one located in the 5' UTR and the other within the 3' UTR. The structure within the 3' UTR has been previously characterised as the 3' cap-independent translation element (3' TE element) and the 5' UTR of barley yellow dwarf virus has been predicted to contain 4 stem loop structures.[1]Mutagenesis showed that stem loop 4 is essential for base pairing with 3'TE and only 5 bases are needed to base pair for mRNA circularization to occur.[2]
References
^Wang S, Browning KS, Miller WA (July 1997). "A viral sequence in the 3'-untranslated region mimics a 5' cap in facilitating translation of uncapped mRNA". The EMBO Journal (Free full text). 16 (13): 4107–4116. doi:10.1093/emboj/16.13.4107. PMC1170033. PMID 9233819.
^Guo L, Allen EM, Miller WA (May 2001). "Base-pairing between untranslated regions facilitates translation of uncapped, nonpolyadenylated viral RNA". Molecular Cell. 7 (5): 1103–1109. doi:10.1016/S1097-2765(01)00252-0. PMID 11389856.
Further reading
Malys N, McCarthy JE (March 2011). "Translation initiation: variations in the mechanism can be anticipated". Cellular and Molecular Life Sciences. 68 (6): 991–1003. doi:10.1007/s00018-010-0588-z. PMID 21076851. S2CID 31720000.
barley, yellow, dwarf, virus, this, article, technical, most, readers, understand, please, help, improve, make, understandable, experts, without, removing, technical, details, july, 2017, learn, when, remove, this, template, message, barley, yellow, dwarf, vir. This article may be too technical for most readers to understand Please help improve it to make it understandable to non experts without removing the technical details July 2017 Learn how and when to remove this template message Barley yellow dwarf virus 5 UTR is a non coding RNA element containing structural elements required for translation of the viral genome Barley yellow dwarf virus 5 UTRConserved secondary structure of BYDV 5 UTR IdentifiersSymbolBYDV 5 UTRRfamRF01508Other dataRNA typecis regulatory elementDomain s barley yellow dwarfPDB structuresPDBeUnlike eukaryotic mRNA this virus lacks a 5 cap and a poly A tail but still circularises its mRNA through base pairing between two stem loops one located in the 5 UTR and the other within the 3 UTR The structure within the 3 UTR has been previously characterised as the 3 cap independent translation element 3 TE element and the 5 UTR of barley yellow dwarf virus has been predicted to contain 4 stem loop structures 1 Mutagenesis showed that stem loop 4 is essential for base pairing with 3 TE and only 5 bases are needed to base pair for mRNA circularization to occur 2 References Edit Wang S Browning KS Miller WA July 1997 A viral sequence in the 3 untranslated region mimics a 5 cap in facilitating translation of uncapped mRNA The EMBO Journal Free full text 16 13 4107 4116 doi 10 1093 emboj 16 13 4107 PMC 1170033 PMID 9233819 Guo L Allen EM Miller WA May 2001 Base pairing between untranslated regions facilitates translation of uncapped nonpolyadenylated viral RNA Molecular Cell 7 5 1103 1109 doi 10 1016 S1097 2765 01 00252 0 PMID 11389856 Further reading EditMalys N McCarthy JE March 2011 Translation initiation variations in the mechanism can be anticipated Cellular and Molecular Life Sciences 68 6 991 1003 doi 10 1007 s00018 010 0588 z PMID 21076851 S2CID 31720000 External links EditPage for Barley yellow dwarf virus 5 UTR at Rfam Retrieved from https en wikipedia org w index php title Barley yellow dwarf virus 5 27UTR amp oldid 1077105350, wikipedia, wiki, book, books, library,
