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BANA test

The BANA test (referring to the enzymatic breakdown of [N-benzoyl-dL-arginine-2-napthylamide]) is used to determine the proteolytic activity of certain oral anaerobes that contribute to oral malodor. Some bacteria, e.g. Prophyromona gingivalis, Treponema denticola, and Bacteroides forsythus (Red complex) produce waste products that are quite odiferous, and as a result contribute to bad breath.[1]

When a sample of a patient's saliva that contains these bacteria is placed within the BANA testing compound, it causes the breakdown of the N-benzoyl enzyme. As a result of this biodegradation occurs, the test compound changes color, indicating a positive reaction.[2]

Uses: Used to identify volatile Sulphur compounds in halitosis patients.

References

  1. ^ Dhalla, N; Patil, S; Chaubey, K. K; Narula, I. S (2015). "The detection of BANA micro-organisms in adult periodontitis before and after scaling and root planing by BANA-Enzymatic™ test kit: An in vivo study". Journal of Indian Society of Periodontology. 19 (4): 401–405. doi:10.4103/0972-124X.154167. PMC 4555797. PMID 26392688.
  2. ^ Bathla, Shalu (2011). Periodontis Revisited. p. 242. ISBN 978-93-5025-367-0.

Further reading

  • Andrade, José Alexandre de; Feres, Magda; Figueiredo, Luciene Cristina de; Salvador, Sérgio Luiz; Cortelli, Sheila Cavalca (June 2010). "The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia". Brazilian Oral Research. 24 (2): 224–230. doi:10.1590/S1806-83242010000200016.
  • Loesche, W. J.; Lopatin, D. E.; Giordano, J.; Alcoforado, G.; Hujoel, P. (1 February 1992). "Comparison of the benzoyl-DL-arginine-naphthylamide (BANA) test, DNA probes, and immunological reagents for ability to detect anaerobic periodontal infections due to Porphyromonas gingivalis, Treponema denticola, and Bacteroides forsythus". Journal of Clinical Microbiology. 30 (2): 427–433. doi:10.1128/JCM.30.2.427-433.1992. PMID 1311335.

bana, test, referring, enzymatic, breakdown, benzoyl, arginine, napthylamide, used, determine, proteolytic, activity, certain, oral, anaerobes, that, contribute, oral, malodor, some, bacteria, prophyromona, gingivalis, treponema, denticola, bacteroides, forsyt. The BANA test referring to the enzymatic breakdown of N benzoyl dL arginine 2 napthylamide is used to determine the proteolytic activity of certain oral anaerobes that contribute to oral malodor Some bacteria e g Prophyromona gingivalis Treponema denticola and Bacteroides forsythus Red complex produce waste products that are quite odiferous and as a result contribute to bad breath 1 When a sample of a patient s saliva that contains these bacteria is placed within the BANA testing compound it causes the breakdown of the N benzoyl enzyme As a result of this biodegradation occurs the test compound changes color indicating a positive reaction 2 Uses Used to identify volatile Sulphur compounds in halitosis patients References Edit Dhalla N Patil S Chaubey K K Narula I S 2015 The detection of BANA micro organisms in adult periodontitis before and after scaling and root planing by BANA Enzymatic test kit An in vivo study Journal of Indian Society of Periodontology 19 4 401 405 doi 10 4103 0972 124X 154167 PMC 4555797 PMID 26392688 Bathla Shalu 2011 Periodontis Revisited p 242 ISBN 978 93 5025 367 0 Further reading EditAndrade Jose Alexandre de Feres Magda Figueiredo Luciene Cristina de Salvador Sergio Luiz Cortelli Sheila Cavalca June 2010 The ability of the BANA test to detect different levels of P gingivalis T denticola and T forsythia Brazilian Oral Research 24 2 224 230 doi 10 1590 S1806 83242010000200016 Loesche W J Lopatin D E Giordano J Alcoforado G Hujoel P 1 February 1992 Comparison of the benzoyl DL arginine naphthylamide BANA test DNA probes and immunological reagents for ability to detect anaerobic periodontal infections due to Porphyromonas gingivalis Treponema denticola and Bacteroides forsythus Journal of Clinical Microbiology 30 2 427 433 doi 10 1128 JCM 30 2 427 433 1992 PMID 1311335 Retrieved from https en wikipedia org w index php title BANA test amp oldid 1054273803, wikipedia, wiki, book, books, library,

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