Anti-Q RNA (formerly Qa RNA) is a small ncRNA from the conjugal plasmid pCF10 of Enterococcus faecalis. It is coded in cis to its regulatory target, prgQ, but can also act in trans. Anti-Q is known to interact with nascent prgQ transcripts to allow formation of an intrinsic terminator, or attenuator, thus preventing transcription of downstream genes.[1][2] This mode of regulation is essentially the same as that of the countertranscript-driven attenuators that control copy number in pT181,[3] pAMbeta1[4] and pIP501[5] and related Staphylococcal plasmids.
Anti-Q is transcribed from the same segment of DNA as prgQ, except from the opposite strand, making it perfectly complementary to a portion of prgQ. Further experiments have experimentally confirmed the original consensus secondary structure and demonstrated that only certain regions of Anti-Q interact with prgQ.[6]
Anti-Q is derived from the 5’ end a longer transcript. The 3’ end of this transcript encodes PrgX, a repressor of prgQ transcription.
Referencesedit
^Bae, T; Kozlowicz BK; Dunny GM (2004). "Characterization of cis-acting prgQ mutants: evidence for two distinct repression mechanisms by Qa RNA and PrgX protein in pheromone-inducible enterococcal plasmid pCF10". Mol Microbiol. 51 (1): 271–281. doi:10.1046/j.1365-2958.2003.03832.x. PMID 14651627. S2CID 26045706.
^Johnson CM, Manias DA, Haemig HA, Shokeen S, Weaver KE, Henkin TM, Dunny GM (2010). "Direct evidence for control of the pheromone-inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript-driven attenuation mechanism". J Bacteriol. 192 (6): 1634–1642. doi:10.1128/JB.01525-09. PMC2832536. PMID 20097859.
^Novick RP, Iordanescu S, Projan SJ, Kornblum J, Edelman I (1989). "pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator". Cell. 59 (2): 395–404. doi:10.1016/0092-8674(89)90300-0. PMID 2478296. S2CID 39261148.
^Le Chatelier E, Ehrlich SD, Jannière L (1996). "Countertranscript-driven attenuation system of the pAM beta 1 repE gene". Mol Microbiol. 20 (5): 1099–1112. doi:10.1111/j.1365-2958.1996.tb02550.x. PMID 8809762. S2CID 27014481.
^Brantl S, Birch-Hirschfeld E, Behnke D (1993). "RepR protein expression on plasmid pIP501 is controlled by an antisense RNA-mediated transcription attenuation mechanism". J Bacteriol. 175 (13): 4052–4061. doi:10.1128/jb.175.13.4052-4061.1993. PMC204834. PMID 8320221.
^Shokeen S, Johnson CM, Greenfield TJ, Manias DA, Dunny GM, E Weaver K (2010). "Structural analysis of the Anti-Q-Qs interaction: RNA-mediated regulation of E. faecalis plasmid pCF10 conjugation". Plasmid. 64 (1): 26–35. doi:10.1016/j.plasmid.2010.03.002. PMC2892192. PMID 20332003.
anti, formerly, small, ncrna, from, conjugal, plasmid, pcf10, enterococcus, faecalis, coded, regulatory, target, prgq, also, trans, anti, known, interact, with, nascent, prgq, transcripts, allow, formation, intrinsic, terminator, attenuator, thus, preventing, . Anti Q RNA formerly Qa RNA is a small ncRNA from the conjugal plasmid pCF10 of Enterococcus faecalis It is coded in cis to its regulatory target prgQ but can also act in trans Anti Q is known to interact with nascent prgQ transcripts to allow formation of an intrinsic terminator or attenuator thus preventing transcription of downstream genes 1 2 This mode of regulation is essentially the same as that of the countertranscript driven attenuators that control copy number in pT181 3 pAMbeta1 4 and pIP501 5 and related Staphylococcal plasmids Anti Q RNAPredicted secondary structure and sequence conservation of Anti Q RNAIdentifiersSymbolAnti Q RNAAlt SymbolsQaRNARfamRF00388Other dataRNA typeGene antisenseDomain s BacteriaSOSO 0000644PDB structuresPDBe Anti Q is transcribed from the same segment of DNA as prgQ except from the opposite strand making it perfectly complementary to a portion of prgQ Further experiments have experimentally confirmed the original consensus secondary structure and demonstrated that only certain regions of Anti Q interact with prgQ 6 Anti Q is derived from the 5 end a longer transcript The 3 end of this transcript encodes PrgX a repressor of prgQ transcription References edit Bae T Kozlowicz BK Dunny GM 2004 Characterization of cis acting prgQ mutants evidence for two distinct repression mechanisms by Qa RNA and PrgX protein in pheromone inducible enterococcal plasmid pCF10 Mol Microbiol 51 1 271 281 doi 10 1046 j 1365 2958 2003 03832 x PMID 14651627 S2CID 26045706 Johnson CM Manias DA Haemig HA Shokeen S Weaver KE Henkin TM Dunny GM 2010 Direct evidence for control of the pheromone inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript driven attenuation mechanism J Bacteriol 192 6 1634 1642 doi 10 1128 JB 01525 09 PMC 2832536 PMID 20097859 Novick RP Iordanescu S Projan SJ Kornblum J Edelman I 1989 pT181 plasmid replication is regulated by a countertranscript driven transcriptional attenuator Cell 59 2 395 404 doi 10 1016 0092 8674 89 90300 0 PMID 2478296 S2CID 39261148 Le Chatelier E Ehrlich SD Janniere L 1996 Countertranscript driven attenuation system of the pAM beta 1 repE gene Mol Microbiol 20 5 1099 1112 doi 10 1111 j 1365 2958 1996 tb02550 x PMID 8809762 S2CID 27014481 Brantl S Birch Hirschfeld E Behnke D 1993 RepR protein expression on plasmid pIP501 is controlled by an antisense RNA mediated transcription attenuation mechanism J Bacteriol 175 13 4052 4061 doi 10 1128 jb 175 13 4052 4061 1993 PMC 204834 PMID 8320221 Shokeen S Johnson CM Greenfield TJ Manias DA Dunny GM E Weaver K 2010 Structural analysis of the Anti Q Qs interaction RNA mediated regulation of E faecalis plasmid pCF10 conjugation Plasmid 64 1 26 35 doi 10 1016 j plasmid 2010 03 002 PMC 2892192 PMID 20332003 External links editPage for Qa RNA at Rfam nbsp This molecular or cell biology article is a stub You can help Wikipedia by expanding it vte Retrieved from https en wikipedia org w index php title Anti Q RNA amp oldid 1150303378, wikipedia, wiki, book, books, library,
