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Alice Vrielink

January 21, 2023

Alice Vrielink is a structural biologist and Professor of Structural Biology in the School of Molecular Sciences at the University of Western Australia. She is known for her work determining the structures of macromolecules such as enzymes and nucleic acids.

Alice Vrielink
Alma materUniversity of London
Scientific career
ThesisThe crystal structure determination of cholesterol oxidase (1989)
Doctoral advisorDavid Mervyn Blow[1]

Education

Vrielink earned a Bachelor of Science in Chemistry and Masters of Science in Physical Chemistry at the University of Calgary in Canada.[2] For her master's research she worked on ligands of angiotensin, a hormone involved in regulating blood pressure.[3] She received a PhD in 1989 from the University of London where she worked on the structure of cholesterol oxidase.[4] She also has a Diploma in Crystallography from the Imperial College of Science and Technology.[2]

Career

Vrielink was an Assistant and Associate Professor at McGill University in Canada from 1994 to 2001.[2] From 2000 until 2007 she served as a Research Professor at the University of California, Santa Cruz and then joined the faculty at University of Western Australia as Professor of Structural Biology in 2007.[2]

Vrielink was a member of the 2014 National Committee on Crystallography[5] She is a past president of the Society of Crystallographers of Australia and New Zealand (SCANZ).[6]

Research

Vrielink conducts research in protein biochemistry and crystallography with a special focus on understanding the structural determinants governing enzyme chemistry.[7] Vrielink's early research centered on the three-dimensional structure of the enzyme cholesterol oxidase first in Brevibacterium[8][9] and then in Streptomyces.[10]

She has been involved in projects that have established the structure of compounds including L-amino-acid oxidase,[11] prions,[12] and snake venom.[13] In 2017, she mapped the molecular structure of EptA,[14] a protein that shields superbugs from antibiotics. This work has been covered by the BBC,[15] ABC,[16] Times Higher Education,[17] The West Australian,[18] and Particle.[19] Subsequent work on EptA has revealed why it may be a good target for drug development.[20][21]

Selected publications

  • Vrielink, A.; Rüger, W.; Driessen, H.P.; Freemont, P.S. (1 August 1994). "Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose". The EMBO Journal. 13 (15): 3413–3422. doi:10.1002/j.1460-2075.1994.tb06646.x. ISSN 0261-4189. PMC 395243. PMID 8062817.
  • Vrielink, Alice; Lloyd, Lesley F; Blow, David M (5 June 1991). "Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 Å resolution". Journal of Molecular Biology. 219 (3): 533–554. doi:10.1016/0022-2836(91)90192-9. ISSN 0022-2836. PMID 2051487.
  • Pawelek, P. D.; Cheah, J.; Coulombe, R.; Macheroux, P.; Ghisla, S.; Vrielink, A. (15 August 2000). "The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site". The EMBO Journal. 19 (16): 4204–4215. doi:10.1093/emboj/19.16.4204. ISSN 0261-4189. PMC 302035. PMID 10944103.
  • Burns, Colin S.; Aronoff-Spencer, Eliah; Dunham, Christine M.; Lario, Paula; Avdievich, Nikolai I.; Antholine, William E.; Olmstead, Marilyn M.; Vrielink, Alice; Gerfen, Gary J.; Peisach, Jack; Scott, William G. (1 March 2002). "Molecular Features of the Copper Binding Sites in the Octarepeat Domain of the Prion Protein". Biochemistry. 41 (12): 3991–4001. doi:10.1021/bi011922x. ISSN 0006-2960. PMC 2905306. PMID 11900542.

References

  1. ^ Vrielink, Alice (3 July 2014). "David Blow (1931-2004) - A Remberance" (PDF). American Crystallographic Association. (PDF) from the original on 3 July 2014. Retrieved 14 October 2021.
  2. ^ a b c d "Researcher Profile: Professor Alice Vrielink". UWA Research Repository.
  3. ^ Vrielink, Alice (1986). Structural and conformational energy studies of ligands of angiotensin converting enzyme and thermolysin (Thesis). Ottawa: National Library of Canada. OCLC 16710356.
  4. ^ Vrielink, Alice; University of London (1989). The crystal structure determination of cholesterol oxidase. hdl:10044/1/47699. OCLC 1063590654.
  5. ^ "Triennial Congress and General Assembly". Australian Academy of Science newsletter.
  6. ^ Vrielink, Alice (2016). "From the President" (PDF). Scanz Newsletter. Retrieved 7 February 2018.
  7. ^ Ducy, Liam. "UWA celebrates Australian link in scientific breakthrough". WAToday, August 20, 2014.
  8. ^ Li, Jiayao; Vrielink, Alice; Brick, Peter; Blow, David M. (26 January 1993). "Crystal structure of cholesterol oxidase complexed with a steroid substrate: Implications for flavin adenine dinucleotide dependent alcohol oxidases". Biochemistry. 32 (43): 11507–11515. doi:10.1021/bi00094a006. ISSN 0006-2960. PMID 8218217.
  9. ^ Vrielink, Alice; Lloyd, Lesley F; Blow, David M (5 June 1991). "Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 Å resolution". Journal of Molecular Biology. 219 (3): 533–554. doi:10.1016/0022-2836(91)90192-9. ISSN 0022-2836. PMID 2051487.
  10. ^ Yue, Q. Kimberley; Kass, Ignatius J.; Sampson, Nicole S.; Vrielink, Alice (1 April 1999). "Crystal Structure Determination of Cholesterol Oxidase from Streptomyces and Structural Characterization of Key Active Site Mutants". Biochemistry. 38 (14): 4277–4286. doi:10.1021/bi982497j. ISSN 0006-2960. PMID 10194345.
  11. ^ Pawelek, P. D. (15 August 2000). "The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site". The EMBO Journal. 19 (16): 4204–4215. doi:10.1093/emboj/19.16.4204. PMC 302035. PMID 10944103.
  12. ^ Burns, Colin S.; Aronoff-Spencer, Eliah; Dunham, Christine M.; Lario, Paula; Avdievich, Nikolai I.; Antholine, William E.; Olmstead, Marilyn M.; Vrielink, Alice; Gerfen, Gary J.; Peisach, Jack; Scott, William G. (1 March 2002). "Molecular Features of the Copper Binding Sites in the Octarepeat Domain of the Prion Protein". Biochemistry. 41 (12): 3991–4001. doi:10.1021/bi011922x. ISSN 0006-2960. PMC 2905306. PMID 11900542.
  13. ^ Kang, Tse Siang; Georgieva, Dessislava; Genov, Nikolay; Murakami, Mário T.; Sinha, Mau; Kumar, Ramasamy P.; Kaur, Punit; Kumar, Sanjit; Dey, Sharmistha; Sharma, Sujata; Vrielink, Alice (2011). "Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis". The FEBS Journal. 278 (23): 4544–4576. doi:10.1111/j.1742-4658.2011.08115.x. ISSN 1742-4658. PMID 21470368. S2CID 40199362.
  14. ^ Anandan, Anandhi; Evans, Genevieve L.; Condic-Jurkic, Karmen; O’Mara, Megan L.; John, Constance M.; Phillips, Nancy J.; Jarvis, Gary A.; Wills, Siobhan S.; Stubbs, Keith A.; Moraes, Isabel; Kahler, Charlene M.; Vrielink, Alice (28 February 2017). "Structure of a lipid A phosphoethanolamine transferase suggests how conformational changes govern substrate binding". Proceedings of the National Academy of Sciences. 114 (9): 2218–2223. doi:10.1073/pnas.1612927114. ISSN 0027-8424. PMC 5338521. PMID 28193899.
  15. ^ Dunlop, Greg (15 February 2017). "Antibiotic resistance: Scientists 'unmask' superbug-shielding protein". BBC News. Retrieved 7 February 2018.
  16. ^ Wildie, Tom (14 February 2017). "Australian scientists make breakthrough in fight against superbugs". ABC News. Retrieved 7 February 2018.
  17. ^ . Times Higher Education. Archived from the original on 8 February 2018. Retrieved 7 February 2018.
  18. ^ O'Leary, Cathy (14 February 2017). "UWA breakthrough in superbug battle". The West Australian. Retrieved 7 February 2018.
  19. ^ Mitchell, Samille (14 March 2017). "WA Scientists Fight Deadly Superbugs". Particle.
  20. ^ Kahler, Charlene M.; Nawrocki, K. L.; Anandan, A.; Vrielink, Alice; Shafer, William M. (2018). "Structure-Function Relationships of the Neisserial EptA Enzyme Responsible for Phosphoethanolamine Decoration of Lipid A: Rationale for Drug Targeting". Frontiers in Microbiology. 9: 1922. doi:10.3389/fmicb.2018.01922. ISSN 1664-302X. PMC 6111236. PMID 30186254.
  21. ^ Anandan, Anandhi; Dunstan, Nicholas W.; Ryan, Timothy M.; Mertens, Haydyn D. T.; Lim, Katherine Y. L.; Evans, Genevieve L.; Kahler, Charlene M.; Vrielink, Alice (1 September 2021). "Conformational flexibility of EptA driven by an interdomain helix provides insights for enzyme–substrate recognition". IUCrJ. 8 (5): 732–746. doi:10.1107/S2052252521005613. ISSN 2052-2525. PMC 8420757. PMID 34584735.

External links

  • Faculty page
  • Lab group page
  • Alice Vrielink publications indexed by Google Scholar

January 21, 2023
alice, vrielink, structural, biologist, professor, structural, biology, school, molecular, sciences, university, western, australia, known, work, determining, structures, macromolecules, such, enzymes, nucleic, acids, alma, materuniversity, londonscientific, c. Alice Vrielink is a structural biologist and Professor of Structural Biology in the School of Molecular Sciences at the University of Western Australia She is known for her work determining the structures of macromolecules such as enzymes and nucleic acids Alice VrielinkAlma materUniversity of LondonScientific careerThesisThe crystal structure determination of cholesterol oxidase 1989 Doctoral advisorDavid Mervyn Blow 1 Contents 1 Education 2 Career 3 Research 4 Selected publications 5 References 6 External linksEducation EditVrielink earned a Bachelor of Science in Chemistry and Masters of Science in Physical Chemistry at the University of Calgary in Canada 2 For her master s research she worked on ligands of angiotensin a hormone involved in regulating blood pressure 3 She received a PhD in 1989 from the University of London where she worked on the structure of cholesterol oxidase 4 She also has a Diploma in Crystallography from the Imperial College of Science and Technology 2 Career EditVrielink was an Assistant and Associate Professor at McGill University in Canada from 1994 to 2001 2 From 2000 until 2007 she served as a Research Professor at the University of California Santa Cruz and then joined the faculty at University of Western Australia as Professor of Structural Biology in 2007 2 Vrielink was a member of the 2014 National Committee on Crystallography 5 She is a past president of the Society of Crystallographers of Australia and New Zealand SCANZ 6 Research EditVrielink conducts research in protein biochemistry and crystallography with a special focus on understanding the structural determinants governing enzyme chemistry 7 Vrielink s early research centered on the three dimensional structure of the enzyme cholesterol oxidase first in Brevibacterium 8 9 and then in Streptomyces 10 She has been involved in projects that have established the structure of compounds including L amino acid oxidase 11 prions 12 and snake venom 13 In 2017 she mapped the molecular structure of EptA 14 a protein that shields superbugs from antibiotics This work has been covered by the BBC 15 ABC 16 Times Higher Education 17 The West Australian 18 and Particle 19 Subsequent work on EptA has revealed why it may be a good target for drug development 20 21 Selected publications EditVrielink A Ruger W Driessen H P Freemont P S 1 August 1994 Crystal structure of the DNA modifying enzyme beta glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose The EMBO Journal 13 15 3413 3422 doi 10 1002 j 1460 2075 1994 tb06646 x ISSN 0261 4189 PMC 395243 PMID 8062817 Vrielink Alice Lloyd Lesley F Blow David M 5 June 1991 Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1 8 A resolution Journal of Molecular Biology 219 3 533 554 doi 10 1016 0022 2836 91 90192 9 ISSN 0022 2836 PMID 2051487 Pawelek P D Cheah J Coulombe R Macheroux P Ghisla S Vrielink A 15 August 2000 The structure of L amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site The EMBO Journal 19 16 4204 4215 doi 10 1093 emboj 19 16 4204 ISSN 0261 4189 PMC 302035 PMID 10944103 Burns Colin S Aronoff Spencer Eliah Dunham Christine M Lario Paula Avdievich Nikolai I Antholine William E Olmstead Marilyn M Vrielink Alice Gerfen Gary J Peisach Jack Scott William G 1 March 2002 Molecular Features of the Copper Binding Sites in the Octarepeat Domain of the Prion Protein Biochemistry 41 12 3991 4001 doi 10 1021 bi011922x ISSN 0006 2960 PMC 2905306 PMID 11900542 References Edit Vrielink Alice 3 July 2014 David Blow 1931 2004 A Remberance PDF American Crystallographic Association Archived PDF from the original on 3 July 2014 Retrieved 14 October 2021 a b c d Researcher Profile Professor Alice Vrielink UWA Research Repository Vrielink Alice 1986 Structural and conformational energy studies of ligands of angiotensin converting enzyme and thermolysin Thesis Ottawa National Library of Canada OCLC 16710356 Vrielink Alice University of London 1989 The crystal structure determination of cholesterol oxidase hdl 10044 1 47699 OCLC 1063590654 Triennial Congress and General Assembly Australian Academy of Science newsletter Vrielink Alice 2016 From the President PDF Scanz Newsletter Retrieved 7 February 2018 Ducy Liam UWA celebrates Australian link in scientific breakthrough WAToday August 20 2014 Li Jiayao Vrielink Alice Brick Peter Blow David M 26 January 1993 Crystal structure of cholesterol oxidase complexed with a steroid substrate Implications for flavin adenine dinucleotide dependent alcohol oxidases Biochemistry 32 43 11507 11515 doi 10 1021 bi00094a006 ISSN 0006 2960 PMID 8218217 Vrielink Alice Lloyd Lesley F Blow David M 5 June 1991 Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1 8 A resolution Journal of Molecular Biology 219 3 533 554 doi 10 1016 0022 2836 91 90192 9 ISSN 0022 2836 PMID 2051487 Yue Q Kimberley Kass Ignatius J Sampson Nicole S Vrielink Alice 1 April 1999 Crystal Structure Determination of Cholesterol Oxidase from Streptomyces and Structural Characterization of Key Active Site Mutants Biochemistry 38 14 4277 4286 doi 10 1021 bi982497j ISSN 0006 2960 PMID 10194345 Pawelek P D 15 August 2000 The structure of L amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site The EMBO Journal 19 16 4204 4215 doi 10 1093 emboj 19 16 4204 PMC 302035 PMID 10944103 Burns Colin S Aronoff Spencer Eliah Dunham Christine M Lario Paula Avdievich Nikolai I Antholine William E Olmstead Marilyn M Vrielink Alice Gerfen Gary J Peisach Jack Scott William G 1 March 2002 Molecular Features of the Copper Binding Sites in the Octarepeat Domain of the Prion Protein Biochemistry 41 12 3991 4001 doi 10 1021 bi011922x ISSN 0006 2960 PMC 2905306 PMID 11900542 Kang Tse Siang Georgieva Dessislava Genov Nikolay Murakami Mario T Sinha Mau Kumar Ramasamy P Kaur Punit Kumar Sanjit Dey Sharmistha Sharma Sujata Vrielink Alice 2011 Enzymatic toxins from snake venom structural characterization and mechanism of catalysis The FEBS Journal 278 23 4544 4576 doi 10 1111 j 1742 4658 2011 08115 x ISSN 1742 4658 PMID 21470368 S2CID 40199362 Anandan Anandhi Evans Genevieve L Condic Jurkic Karmen O Mara Megan L John Constance M Phillips Nancy J Jarvis Gary A Wills Siobhan S Stubbs Keith A Moraes Isabel Kahler Charlene M Vrielink Alice 28 February 2017 Structure of a lipid A phosphoethanolamine transferase suggests how conformational changes govern substrate binding Proceedings of the National Academy of Sciences 114 9 2218 2223 doi 10 1073 pnas 1612927114 ISSN 0027 8424 PMC 5338521 PMID 28193899 Dunlop Greg 15 February 2017 Antibiotic resistance Scientists unmask superbug shielding protein BBC News Retrieved 7 February 2018 Wildie Tom 14 February 2017 Australian scientists make breakthrough in fight against superbugs ABC News Retrieved 7 February 2018 Stopping the rise of the superbug Times Higher Education Archived from the original on 8 February 2018 Retrieved 7 February 2018 O Leary Cathy 14 February 2017 UWA breakthrough in superbug battle The West Australian Retrieved 7 February 2018 Mitchell Samille 14 March 2017 WA Scientists Fight Deadly Superbugs Particle Kahler Charlene M Nawrocki K L Anandan A Vrielink Alice Shafer William M 2018 Structure Function Relationships of the Neisserial EptA Enzyme Responsible for Phosphoethanolamine Decoration of Lipid A Rationale for Drug Targeting Frontiers in Microbiology 9 1922 doi 10 3389 fmicb 2018 01922 ISSN 1664 302X PMC 6111236 PMID 30186254 Anandan Anandhi Dunstan Nicholas W Ryan Timothy M Mertens Haydyn D T Lim Katherine Y L Evans Genevieve L Kahler Charlene M Vrielink Alice 1 September 2021 Conformational flexibility of EptA driven by an interdomain helix provides insights for enzyme substrate recognition IUCrJ 8 5 732 746 doi 10 1107 S2052252521005613 ISSN 2052 2525 PMC 8420757 PMID 34584735 External links EditFaculty page Lab group page Vrielink s obituary for David Blow her Ph D advisor Alice Vrielink publications indexed by Google Scholar Retrieved from https en wikipedia org w index php title Alice Vrielink amp oldid 1108932081, wikipedia, wiki, book, books, library,

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