2-hydroxy-6-oxonona-2,4-dienedioate hydrolase

2-hydroxy-6-oxonona-2,4-dienedioate hydrolase (EC 3.7.1.14, mhpC (gene)) is an enzyme with systematic name (2Z,4E)-2-hydroxy-6-oxona-2,4-dienedioate succinylhydrolase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction:

1. (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H₂O ${\displaystyle \rightleftharpoons }$ (2Z)-2-hydroxypenta-2,4-dienoate + succinate
2. (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H₂O ${\displaystyle \rightleftharpoons }$ (2Z)-2-hydroxypenta-2,4-dienoate + fumarate

2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
Identifiers
EC no.	3.7.1.14
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

This enzyme catalyses a step in a pathway of phenylpropanoid compounds degradation.

References

1. Burlingame R, Chapman PJ (July 1983). "Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli". Journal of Bacteriology. 155 (1): 113–21. PMC 217659. PMID 6345502.
2. Burlingame RP, Wyman L, Chapman PJ (October 1986). "Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation". Journal of Bacteriology. 168 (1): 55–64. PMC 213419. PMID 3531186.
3. Lam WW, Bugg TD (1994). "Chemistry of extradiol aromatic ring cleavage: isolation of a stable dienol ring fission intermediate and stereochemistry of its enzymatic hydrolytic cleavage". J. Chem. Soc., Chem. Commun. 10 (10): 1163–1164. doi:10.1039/c39940001163.
4. Lam WW, Bugg TD (October 1997). "Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase". Biochemistry. 36 (40): 12242–51. doi:10.1021/bi971115r. PMID 9315862.
5. Ferrández A, Garciá JL, Díaz E (April 1997). "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12". Journal of Bacteriology. 179 (8): 2573–81. PMC 179006. PMID 9098055.
6. Díaz E, Ferrández A, García JL (June 1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12". Journal of Bacteriology. 180 (11): 2915–23. PMC 107259. PMID 9603882.

External links

• 2-hydroxy-6-oxonona-2,4-dienedioate+hydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)